Dataset Information

Stage-specific protein regulation during somatic embryo development of Carica papaya L. ‘Golden’

ABSTRACT: Somatic embryogenesis is an important biotechnological technique for large-scale propagation of elite genotypes. Correlations of stage-specific compounds associated with somatic embryo development can help elucidate the ontogenesis of Carica papaya L. somatic embryos and improve tissue culture protocols. To identify the stage-specific proteins that are present during the differentiation of C. papaya somatic embryos, proteomic analyses of embryos at the globular, heart, torpedo and cotyledonary stages were performed. Comparative proteomic analysis revealed a total of 801 proteins, 392 of which were classified as differentially accumulated proteins in at least one of the developmental stages. The globular stage presented a higher number of unique proteins (16), 7 of which were isoforms of 60S ribosomal proteins, suggesting high translational activity at the beginning of somatic embryogenesis. Proteins related to mitochondrial metabolism accumulated to a high degree at the early developmental stages, after which they decreased with increasing development, contributing to cell homeostasis in early somatic embryos. On the other hand, a progressive increase in the accumulation of vicilin, late embryogenesis abundant proteins and chloroplastic proteins leading to somatic embryo maturation was observed. Additionally, the differential accumulation of acetylornithine deacetylase and S-adenosylmethionine synthase 2 proteins correlated with increased contents of putrescine and spermidine, suggesting that polyamine metabolism is important to somatic embryo development. Taken together, the results showed that somatic embryo development in C. papaya is regulated by the differential accumulation of proteins, with ribosomal and mitochondrial proteins being more abundant during the early somatic embryo stages, while proteins involved in seed maturation are more abundant during the late stages.

INSTRUMENT(S): SYNAPT G2-Si

ORGANISM(S): Carica Papaya

TISSUE(S): Plant Cell, Somatic Embryo

SUBMITTER: Vanildo Silveira

LAB HEAD: Vanildo Silveira

PROVIDER: PXD021107 | Pride | 2020-11-12

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2018_09_20_PAPAYA_NADIA_001.raw.zip	Raw
	2018_09_20_PAPAYA_NADIA_002.raw.zip	Raw
	2018_09_20_PAPAYA_NADIA_003.raw.zip	Raw
	2018_09_20_PAPAYA_NADIA_004.raw.zip	Raw
	2018_09_20_PAPAYA_NADIA_005.raw.zip	Raw

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Publications

Stage-specific protein regulation during somatic embryo development of Carica papaya L. 'Golden'.

Botini Nadia N Almeida Felipe Astolpho FA Cruz Kaliane Zaira Camacho Maximiano KZCM Reis Ricardo Souza RS Vale Ellen Moura EM Garcia Ana Beatriz AB Santa-Catarina Claudete C Silveira Vanildo V

Biochimica et biophysica acta. Proteins and proteomics 20201106 2

Somatic embryogenesis is an important biotechnological technique for large-scale propagation of elite genotypes. Identifying stage-specific compounds associated with somatic embryo development can help elucidate the ontogenesis of Carica papaya L. somatic embryos and improve tissue culture protocols. To identify the stage-specific proteins that are present during the differentiation of C. papaya somatic embryos, proteomic analyses of embryos at the globular, heart, torpedo and cotyledonary devel ...[more]

PMID: 33161157

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