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RBPome of primary human T helper cells

ABSTRACT: Post-transcriptional gene regulation is complex, dynamic and ensures proper T cell function. The targeted transcripts can simultaneously respond to various factors as evident for Icos, an mRNA regulated by several RNA binding proteins (RBPs), including Roquin. However, fundamental information about the entire RBPome involved in post-transcriptional gene regulation in T cells is lacking. Here, we applied global RNA interactome capture (RNA-IC) and orthogonal organic phase separation (OOPS) to human and mouse primary T cells and identified the core T cell RBPome. This defined 798 mouse and 801 human proteins as RBPs, unexpectedly containing signaling proteins like Stat1, Stat4 and Vav1. The data represent an atlas of RNA-binding proteins in human and mouse T helper cells as a resource for studying higher order post-transcriptional gene regulation.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Cell Culture

SUBMITTER: Michael Wierer

LAB HEAD: Michael Wierer

PROVIDER: PXD021169 | Pride | 2021-07-01

REPOSITORIES: Pride

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			Action	DRS
	Human_CL_interphase_1.raw	Raw
	Human_CL_interphase_2.raw	Raw
	Human_CL_interphase_3.raw	Raw
	Human_CL_interphase_4.raw	Raw
	Human_CL_organic_RNAse_1.raw	Raw

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RBPome of primary mouse T helper cells

Project description:Post-transcriptional gene regulation is complex, dynamic and ensures proper T cell function. The targeted transcripts can simultaneously respond to various factors as evident for Icos, an mRNA regulated by several RNA binding proteins (RBPs), including Roquin. However, fundamental information about the entire RBPome involved in post-transcriptional gene regulation in T cells is lacking. Here, we applied global RNA interactome capture (RNA-IC) and orthogonal organic phase separation (OOPS) to human and mouse primary T cells and identified the core T cell RBPome. This defined 798 mouse and 801 human proteins as RBPs, unexpectedly containing signaling proteins like Stat1, Stat4 and Vav1. The data represent an atlas of RNA-binding proteins in human and mouse T helper cells as a resource for studying higher order post-transcriptional gene regulation.

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Project description:Post-transcriptional gene regulation in T cells is dynamic and complex as targeted transcripts respond to various factors. This becomes evident for the Icos mRNA encoding an essential costimulatory receptorthatexhibits coordinate regulation by several RNA-binding proteins (RBPs), including Roquin-1 and Roquin-2. Utilizing global RNA interactome capture (RNA-IC) and orthogonal organic phase separation (OOPS) we identify a core RBPome of 798 mouse and 801 human T cell proteins. The RBPome also contained Stat1, Stat4 and Vav1 proteins suggesting unexpected functions for these transcription factors and signal transducers. Based on proximity to Roquin-1, we selected ~50 RBPs for testing coregulation of Roquin-1/2 targets by induced expression in wild-type or Roquin-1/2-deficient T cells. Besides Roquin-independent contributions from Rbms1 and Cpeb4 we also unraveled Roquin-1/2-dependent and target-specific coregulation of Icos byCelf1 and Igf2bp3. These findings define the cellular RBPome as post-transcriptional context that predetermines the extent of target regulation by individual trans-acting factors.

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