Dataset Information

Alkaloids modify the poison frog proteome

ABSTRACT: Poison frogs sequester chemical defenses from their diet of leaf litter arthropods for defense against predation. Little is known about the physiological adaptations that confer this unusual bioaccumulation ability. We conducted an alkaloid-feeding experiment with the Diablito poison frog (Oophaga sylvatica) to determine how quickly alkaloids are accumulated and how toxins modify frog physiology using quantitative proteomics. Diablito frogs rapidly accumulated the alkaloid decahydroquinoline within four days, and dietary alkaloid exposure modified protein abundance in the intestines, liver, and skin. Many proteins that increased in abundance with toxin accumulation are plasma glycoproteins, including the complement system and the toxin-binding protein saxiphilin. Other protein classes that change in abundance with toxin accumulation are membrane proteins involved in small molecule transport and metabolism. Overall, this work shows poison frogs can rapidly accumulate alkaloids, which alter carrier protein abundance, initiate an immune response, and alter small molecule transport and metabolism dynamics across tissues

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Oophaga Sylvatica

TISSUE(S): Intestine, Liver, Skin

SUBMITTER: Jeremy OConnell

LAB HEAD: Lauren A. O’Connell

PROVIDER: PXD021216 | Pride | 2021-01-25

REPOSITORIES: Pride

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			Action	DRS
	332529_m10821_vertPHROG.mzId.gz	Mzid
	332530_m10822_vertPHROG.mzId.gz	Mzid
	332531_m10823_vertPHROG.mzId.gz	Mzid
	332532_m10824_vertPHROG.mzId.gz	Mzid
	332533_m10825_vertPHROG.mzId.gz	Mzid

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Publications

Rapid toxin sequestration modifies poison frog physiology.

O'Connell Lauren A LA O'Connell Jeremy D JD Paulo Joao A JA Trauger Sunia A SA Gygi Steven P SP Murray Andrew W AW

The Journal of experimental biology 20210209 Pt 3

Poison frogs sequester chemical defenses from their diet of leaf litter arthropods for defense against predation. Little is known about the physiological adaptations that confer this unusual bioaccumulation ability. We conducted an alkaloid-feeding experiment with the Diablito poison frog (<i>Oophaga sylvatica</i>) to determine how quickly alkaloids are accumulated and how toxins modify frog physiology using quantitative proteomics. Diablito frogs rapidly accumulated the alkaloid decahydroquinol ...[more]

PMID: 33408255

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