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Quantitative proteomics reveals dual effects of calcium on radicle protrusion in soybean

ABSTRACT: To better elucidate the mechanisms of calcium-mediated germination of soybean seed, morphological analysis was performed in seed imbibed with different concentrations of CaCl2. Based on morphological results, radicle of germinating seed imbibed with 5 and 50 mM CaCl2 was collected for proteomic analysis.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Glycine Max

TISSUE(S): Plant Cell, Root

DISEASE(S): Disease Free

SUBMITTER: Xin WANG

LAB HEAD: Shun-Li Zhou

PROVIDER: PXD021238 | Pride | 2020-10-19

REPOSITORIES: Pride

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Quantitative proteomics reveals dual effects of calcium on radicle protrusion in soybean.

Wang Xin X Hu Han H Li Fang F Yang Bingxian B Komatsu Setsuko S Zhou Shunli S

Journal of proteomics 20201003

To reveal calcium-mediated germination in soybean, a gel-free/label-free proteomics was performed in radicle of seed imbibed with CaCl2. Morphological analysis presented promoting and suppressing performance of seed growth under 5 and 50 mM CaCl2, respectively. A total of 106 and 581 proteins were identified in response to 5 and 50 mM CaCl2, respectively. Among 33 proteins, which were simultaneously affected by 5 and 50 mM CaCl2 imbibition, proteins re ...[more]

PMID: 33017647

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