Dataset Information

Characterisation of the adhesome of patient-derived cutaneous squamous carcinoma cells

ABSTRACT: Interactions between cells and the extracellular matrix, mediated by integrin adhesion complexes (IACs), play key roles in cancer progression and metastasis. We report here systems-level changes in the adhesome during progression of a patient-derived cutaneous squamous cell carcinoma (cSCC). We found that the actin regulatory protein Mena is enriched in IACs in metastatic cSCC cells and is connected within a subnetwork of actin-binding proteins to the LINC complex component nesprin-2.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skin

DISEASE(S): Skin Squamous Cell Carcinoma

SUBMITTER: Adam Byron

LAB HEAD: Margaret Frame

PROVIDER: PXD021492 | Pride | 2023-03-25

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	F151028_01_QE2_jbwills_P_PF_120_M1r1-RD1.raw	Raw
	F151028_02_QE2_jbwills_P_PF_120_M1r1-RD1.raw	Raw
	F151028_03_QE2_jbwills_P_PF_120_M1r2-RD2.raw	Raw
	F151028_04_QE2_jbwills_P_PF_120_M1r2-RD2.raw	Raw
	F151028_05_QE2_jbwills_P_PF_120_M1r4-RD3.raw	Raw

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Publications

Mena regulates nesprin-2 to control actin-nuclear lamina associations, trans-nuclear membrane signalling and gene expression.

Li Mow Chee Frederic F Beernaert Bruno B Griffith Billie G C BGC Loftus Alexander E P AEP Kumar Yatendra Y Wills Jimi C JC Lee Martin M Valli Jessica J Wheeler Ann P AP Armstrong J Douglas JD Parsons Maddy M Leigh Irene M IM Proby Charlotte M CM von Kriegsheim Alex A Bickmore Wendy A WA Frame Margaret C MC Byron Adam A

Nature communications 20230323 1

Interactions between cells and the extracellular matrix, mediated by integrin adhesion complexes, play key roles in fundamental cellular processes, including the sensing and transduction of mechanical cues. Here, we investigate systems-level changes in the integrin adhesome in patient-derived cutaneous squamous cell carcinoma cells and identify the actin regulatory protein Mena as a key node in the adhesion complex network. Mena is connected within a subnetwork of actin-binding proteins to the L ...[more]

PMID: 36959177

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