Proteomics

Dataset Information

0

CD63/CD9-GFP extracellular vesicles LC-MS/MS


ABSTRACT: This study aimed to identify subpopulations of extracellular vesicles (EVs) secreted by HeLa cells, and determine markers which enable to identify them, and which indicate their endosomal or plasma membrane origin. We used CD63 and CD9 as markers of EVs, and followed their intracellular trafficking using the RUSH assay. We used mass spectrometry to identify specific or common proteins in immunoprecipitated GFP+ EVs from HeLa transfected with the RUSH CD63-GFP or CD9-GFP, after a short (3h) or a long (24h) trafficking time from the endoplasmic reticulum.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Extracellular Vesicle, Cell Culture

SUBMITTER: Valentin SABATET  

LAB HEAD: Damarys Loew

PROVIDER: PXD021515 | Pride | 2021-05-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Design.xls Xls
H3341FD.msf Msf
H3341FD.raw Raw
H3342FD.msf Msf
H3342FD.raw Raw
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Publications


Despite their roles in intercellular communications, the different populations of extracellular vesicles (EVs) and their secretion mechanisms are not fully characterized: how and to what extent EVs form as intraluminal vesicles of endocytic compartments (exosomes), or at the plasma membrane (PM) (ectosomes) remains unclear. Here we follow intracellular trafficking of the EV markers CD9 and CD63 from the endoplasmic reticulum to their residency compartment, respectively PM and late endosomes. We  ...[more]

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