Dataset Information

Dietary protein in vitro digest (LC-MS)

ABSTRACT: 16 dietary protein preparations were digested according to a standardized invitro digestion (InfoGest) protocol, simulating gastrointestinal digestion. Resulting oligopeptide mixtures were subsequently analyzed by UPLC-MS using DDA shot-gun detection. Peak intensity data from MS1 spectra were obtained using Progenesis QI. MS1 peak data were matched and filtered based on overlap with the reference digest of Whey protein using a custom made script in R. MSMS fragmentation spectra were converted to .mgf format and de novo interpreted using SearchGUI 3.3.17 and RapidNOVOR algorithm. Raw data, peak intensity tables and de novo interpretaion data are provided.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bos Taurus (bovine) Gallus Gallus (chicken) Pisum Sativum (garden Pea) Solanum Tuberosum (potato) Saccharomyces Cerevisiae (baker's Yeast) Zea Mays (maize) Triticum Aestivum (wheat)

TISSUE(S): Egg

SUBMITTER: Antoine America

LAB HEAD: Antoine H.P. America

PROVIDER: PXD021577 | Pride | 2021-09-09

REPOSITORIES: Pride

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Publications

A combined microphysiological-computational omics approach in dietary protein evaluation.

Jochems Paulus G M PGM Keusters Willem R WR America Antoine H P AHP Rietveld Pascale C S PCS Bastiaan-Net Shanna S Ariëns Renata M C RMC Tomassen Monic M M MMM Lewis Fraser F Li Yang Y Westphal Koen G C KGC Garssen Johan J Wichers Harry J HJ van Bergenhenegouwen Jeroen J Masereeuw Rosalinde R

NPJ science of food 20201217 1

Food security is under increased pressure due to the ever-growing world population. To tackle this, alternative protein sources need to be evaluated for nutritional value, which requires information on digesta peptide composition in comparison to established protein sources and coupling to biological parameters. Here, a combined experimental and computational approach is presented, which compared seventeen protein sources with cow's whey protein concentrate (WPC) as the benchmark. In vitro diges ...[more]

PMID: 33335099

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