Dataset Information

Anammox enrichment culture glycoproteomics

ABSTRACT: The physiology of the planctomycetal anammox bacteria makes them particularly special because they share features with all three domains of life. Anammox bacteria have been reported recently to produce surface-layer proteins, which represent the outermost layer and provide structure, shape and protection under extreme conditions. Furthermore, we report on the unique cell surface-layer glycosylation of the anammox bacterium Ca. Kuenenia stuttgartiensis as revealed by a newly established glycoproteomics approach. This approach enables untargeted exploration of prokaryotic protein glycosylation from (high-resolution) shotgun proteomics data directly.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Candidatus Brocadia Sapporoensis Candidatus Kuenenia Stuttgartiensis

SUBMITTER: Martin Pabst

LAB HEAD: Dr. Martin Pabst

PROVIDER: PXD021600 | Pride | 2021-08-05

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MP09112018_Broc_ML_2hrs_gps_method_DDA01.mzXML	Mzxml
	MP09112018_Broc_ML_2hrs_gps_method_DDA01.raw	Raw
	MP09112018_Broc_ML_2hrs_gps_method_DDA01_BROC_MF_44.csv	Csv
	MP09112018_Broc_ML_2hrs_gps_method_DDA01_BROC_UB_75.csv	Csv
	MP09112018_Ecoli_2hrs_gps_method_DDA01.mzXML	Mzxml

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Publications

A general approach to explore prokaryotic protein glycosylation reveals the unique surface layer modulation of an anammox bacterium.

Pabst Martin M Grouzdev Denis S DS Lawson Christopher E CE Kleikamp Hugo B C HBC de Ram Carol C Louwen Rogier R Lin Yue Mei YM Lücker Sebastian S van Loosdrecht Mark C M MCM Laureni Michele M

The ISME journal 20210802 2

The enormous chemical diversity and strain variability of prokaryotic protein glycosylation makes their large-scale exploration exceptionally challenging. Therefore, despite the universal relevance of protein glycosylation across all domains of life, the understanding of their biological significance and the evolutionary forces shaping oligosaccharide structures remains highly limited. Here, we report on a newly established mass binning glycoproteomics approach that establishes the chemical iden ...[more]

PMID: 34341504

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