Proteomics

Dataset Information

0

TMTPro Complementary Ion Quantification Increase Plexing and Sensitivity for Accurate Multiplexed Proteomics at the MS2 level


ABSTRACT: The ability of the TMTPro isobaric labeling reagents to form complementary ions for accurate multiplexed proteomics at the MS2 level was investigated. Human and yeast peptides were labeled in distinct ratios to analyze the effect of interference on the quantification accuracy. A method, TMTProC, was developed and optimized for accurate, sensitive MS2-level quantification of up to 8 conditions in one MS-run.

INSTRUMENT(S): Orbitrap Eclipse, Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Michael Stadlmeier  

LAB HEAD: Martin Wühr

PROVIDER: PXD021661 | Pride | 2021-05-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
TGR_08290.raw Raw
TGR_08290_TMT0_Hela_Lance_11_10_19_TMTcplus.mgf Mgf
TGR_08290_TMT0_Hela_Lance_11_10_19_TMTcplus.mzid.gz Mzid
TGR_08914.raw Raw
TGR_08914_HeLa_TMTPro0std_20_03_20_InjTimeOpt_TMTc.mgf Mgf
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Publications

TMTpro Complementary Ion Quantification Increases Plexing and Sensitivity for Accurate Multiplexed Proteomics at the MS2 Level.

Johnson Alex A   Stadlmeier Michael M   Wühr Martin M  

Journal of proteome research 20210430 6


Multiplexed proteomics is a powerful tool to assay cell states in health and disease, but accurate quantification of relative protein changes is impaired by interference from co-isolated peptides. Interference can be reduced by using MS3-based quantification, but this reduces sensitivity and requires specialized instrumentation. An alternative approach is quantification by complementary ions, the balancer group-peptide conjugates, which allows accurate and precise multiplexed quantification at t  ...[more]

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