Proteomics

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Proteomics reveals virulence-related proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii yeast cells grown in the presence of L-dihydroxyphenylalanine


ABSTRACT: Species of the genus Paracoccidioides cause a systemic infection in human patients. Yeast cells of Paracoccidioides spp. produce melanin in the presence of L-dihydroxyphenylalanine and during infection, which may impact the pathogen survival into the host. To better understand the metabolic changes that occur in melanized Paracoccidioides spp. cells, a proteomic approach was performed to compare melanized and non-melanized Paracoccidioides brasiliensis and Paracoccidioides lutzii yeast cells. Melanization was conducted using L-dihydroxyphenylalanine as a precursor and quantitative proteomics was performed using reversed-phase chromatography coupled to high resolution mass spectrometry. When comparing melanized versus non-melanized cells, 999 and 577 differentially abundant proteins were identified for P. brasiliensis and P. lutzii, respectively. Functional enrichment and comparative analysis revealed 30 abundant biological processes in melanized P. brasiliensis and 18 in P. lutzii, while non-melanized cells from these species had 21 and 25 differentially abundant processes, respectively. Melanized cells presented abundance of other virulence-associated proteins, such as phospholipase, proteases, superoxide dismutase, heat-shock proteins, as well as proteins related to cell-wall remodeling and vesicular transport. The results suggest that L-dihydroxyphenilalanine increases virulence of Paracoccidioides spp. through a complex mechanism involving not only melanin, but other virulence factors as well.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Fungi Paracoccidioides Brasiliensis (strain Pb18) Paracoccidioides Lutzii (strain Atcc Mya-826 / Pb01) (paracoccidioides Brasiliensis)

TISSUE(S): Cell Culture, Vegetative Cell (sensu Fungi)

SUBMITTER: Richard Hemmi Valente  

LAB HEAD: Rodrigo Almeida-Paes

PROVIDER: PXD021711 | Pride | 2021-01-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20190801_Pb18LD_01_Run01.raw Raw
20190801_Pb18LD_01_Run01.sqt Other
20190801_Pb18LD_01_Run02.raw Raw
20190801_Pb18LD_01_Run02.sqt Other
20190801_Pb18LD_01_Run03.raw Raw
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Publications

Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in <i>Paracoccidioides</i> <i>brasiliensis</i> and <i>Paracoccidioides</i> <i>lutzii</i> Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine.

Almeida-Paes Rodrigo R   Almeida Marcos Abreu MA   Baeza Lilian Cristiane LC   Marmello Leticia Andrade Mendes LAM   Trugilho Monique Ramos de Oliveira MRO   Nosanchuk Joshua Daniel JD   Soares Celia Maria de Almeida CMA   Valente Richard Hemmi RH   Zancopé-Oliveira Rosely Maria RM  

Journal of fungi (Basel, Switzerland) 20201201 4


Species of the genus <i>Paracoccidioides</i> cause a systemic infection in human patients. Yeast cells of <i>Paracoccidioides</i> spp. produce melanin in the presence of L-dihydroxyphenylalanine and during infection, which may impact the pathogen's survival in the host. To better understand the metabolic changes that occur in melanized <i>Paracoccidioides</i> spp. cells, a proteomic approach was performed to compare melanized and non-melanized <i>Paracoccidioides brasiliensis</i> and <i>Paracocc  ...[more]

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