Dataset Information

Profile of growth factors in calf costochondral junction – the site of initial bone formation

ABSTRACT: Endochondral bone formation is orchestrated by growth factors produced by chondrocytes and deposited in cartilage matrix. Some of these factors were identified, but their complete list and relationship remains unknown. In this work the growth factors were isolated from calcified cartilage of costochondral junctions. The isolation involved hyaluronidase digestion, guanidinium hydrochloride (GuHCl) extraction, HCl decalcification and GuHCl extraction of decalcified matrix. Growth factors were purified by heparin chromatography and estimated by enzyme-linked immunosorbent assay (ELISA) or used for protein sequence analysis. Bone morphogenetic protein-7 (BMP-7), Growth/differentiation factor 5 (GDF-5), and NEL-like protein 1 (NELL-1) quantitatively dominated in material obtained in all steps of isolation. During ossification perivascular cells and septoclasts enter the cartilage and release growth factors stimulating osteoclastogenesis. Osteoclasts dissolve calcified cartilage and transport released factors needed for stimulation of osteoprogenitor cells. Presence of BMP-7, GDF-5 and NELL-1 at the site of initial bone formation may suggest that their synergistic action favors osteogenesis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Cartilage

SUBMITTER: Michał Kistowski

LAB HEAD: Michał Dadlez

PROVIDER: PXD021781 | Pride | 2021-05-03

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	80427835iwa_osadkoncowy1.raw	Raw
	80427836iwa_osadkoncowy2.raw	Raw
	806091373iwan_osadkoncowy3.raw	Raw
	806091374iwan_plynkoncowy3.raw	Raw
	F113689.mgf	Mgf

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Publications

Growth factor profile in calcified cartilage from the metaphysis of a calf costochondral junction, the site of initial bone formation.

Iwan Anna A Moskalewski Stanisław S Hyc Anna A

Biomedical reports 20210409 6

Endochondral bone formation is orchestrated by growth factors produced by chondrocytes and deposited in the cartilage matrix. Whilst some of these factors have been identified, the complete list and their relationship remains unknown. In the present study, the growth factors were isolated from non-calcified and calcified cartilage of costochondral junctions. Cartilage dissected from the ribs of 6-20-week-old calves was purchased from a local butcher within 24 h of the death of the animal. The is ...[more]

PMID: 33884197

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