Dataset Information

Enhancing the chondrogenic potential of chondrogenic progenitor cells by deletion of RAB5C

ABSTRACT: Osteoarthritis (OA) is the most prevalent chronic joint disease that affects a majority of the elderly. Chondrogenic progenitor cells (CPCs) reside in late stage OA cartilage tissue, producing a fibrocartilagenous extra-cellular matrix and can be manipulated in-vitro, to deposit proteins of healthy articular cartilage. CPCs are under control of SOX9 and RUNX2 and in order to enhance their chondrogenic potential, we found that RUNX2 plays a pivotal role in chondrogenesis. In another approach, CPCs carrying a knockout of RAB5C, a protein involved in endosomal trafficking, demonstrated elevated expression of various chondrogenic markers including the SOX trio and displayed an increased COL2 deposition, whereas no changes of COL1 deposition was observed. We report RAB5C as an attractive target for future therapeutic approaches to increase the COL2 content in the diseased joint.

INSTRUMENT(S): TripleTOF 5600, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cartilage Tissue, Cell Culture

DISEASE(S): Osteoarthritis

SUBMITTER: Christof Lenz

LAB HEAD: Christof Lenz

PROVIDER: PXD021785 | Pride | 2021-09-09

REPOSITORIES: Pride

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			Action	DRS
	G_Cingoez_280714_050914_R1_L1_01.raw	Raw
	G_Cingoez_280714_050914_R1_L1_02.raw	Raw
	G_Cingoez_280714_050914_R1_L1_03.raw	Raw
	G_Cingoez_280714_050914_R1_L1_04.raw	Raw
	G_Cingoez_280714_050914_R1_L1_05.raw	Raw

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Enhancing the chondrogenic potential of chondrogenic progenitor cells by deleting RAB5C.

Janssen Jerome Nicolas JN Izzi Valerio V Henze Elvira E Cingöz Gökhan G Lowen Florian F Küttner David D Neumann Ruth R Lenz Christof C Rosen Vicki V Miosge Nicolai N

iScience 20210422 5

Osteoarthritis (OA) is the most prevalent chronic joint disease that affects a large proportion of the elderly population. Chondrogenic progenitor cells (CPCs) reside in late-stage OA cartilage tissue, producing a fibrocartilaginous extracellular matrix; these cells can be manipulated <i>in vitro</i> to deposit proteins of healthy articular cartilage. CPCs are under the control of SOX9 and RUNX2. In our earlier studies, we showed that a knockdown of RUNX2 enhanced the chondrogenic potential of C ...[more]

PMID: 34013174

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