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Yellow nutsedge co-opts a seed-like proteome in oil rich tubers

ABSTRACT: Drought is the prime stressors that land plants have to overcome since their dawn. One strategy that land plants employ is to limit water loss and to transport water via a vascular system, another is to produce desiccation tolerant cells and tissues. In most flowering plants, the latter strategy is limited to seeds and to some extend pollen. Common to desiccation and drought tolerance is the accumulation of small osmolytes and proteins with protective functions. Also common to both strategies is the accumulation of neutral lipids, foremost triacylglycerol (TAG), in cytosolic lipid droplets (LDs) with especially high levels being reached in embryonic tissues. Here, we investigated yellow nutsedge (Cyperus esculentus), a monocot, perennial C4 plant. This species produces stolon-derived underground tubers that can fully desiccate and remain viable for years. Yellow nutsedge stands out, as its tubers store 25-30 % of their dry mass in lipids especially TAG, similar to seeds. We generated nanoLC-MS/MS-based proteomes in five replicates of four stages of tuber development and compared them to the proteomes of roots and leaves, yielding 2257 distinct protein groups. Our data reveal a striking upregulation of hallmark proteins of seeds in the tubers. A deeper comparison to a previously published proteome of Arabidopsis seeds and seedlings indicate that indeed a seed-like proteome was co-opted. This was further supported by an analysis of the proteome of a lipid-droplet enriched fraction of yellow nutsedge, which also displayed seed-like characteristics.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Cyperus Esculentus Var. Sativus

TISSUE(S): Root, Tuber, Leaf

SUBMITTER: Till Ischebeck

LAB HEAD: Till Ischebeck

PROVIDER: PXD021894 | Pride | 2022-10-31

REPOSITORIES: Pride

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Project description:To better undersand the effects of drought stress on wheat developing seeds, the transcription profile of early developing wheat seeds under control and drought stress conditions were comparatively analyzed by using the Affymetrix wheat geneChip. Drought stress is a major yield-limiting factor for wheat. Wheat yields are particularly sensitive to drought stress during reproductive development. Early seed development stage is an important determinant of seed size, one of the yield components. We specifically examined the impact of drought stress imposed during postzygotic early seed development in wheat. We imposed a short-term drought stress on plants with day-old seeds and observed that even a short-duration drought stress significantly reduced the size of developing seeds as well as mature seeds. Drought stress delayed the developmental transition from syncytial to cellularized stage of endosperm. Coincident with reduced seed size and delayed endosperm development, a subset of genes associated with cytoskeleton organization was misregulated in developing seeds under drought-stressed. Several genes linked to hormone pathways were also differentially regulated in response to drought stress in early seeds. Notably, drought stress strongly repressed the expression of wheat storage protein genes such as gliadins, glutenins and avenins as early as 3 days after pollination. Our results provide new insights on how some of the early seed developmental events are impacted by water stress, and the underlying molecular pathways that can possibly impact both grain size and quality in wheat. Winter wheat cultivar Redland, PI 502907 (Triticum aestivum L.) was used for this study. Seedlings were vernalized at 4°C for 6 weeks and then transplanted to a one gallon pot of soil-sand mixture (3:1, v/v) and grown in a growth chamber under the following conditions: relative humidity, 50–70%; 16-h light/8-h dark photoperiod; 21°C daytime temperature and 18°C nights. Plants were watered regularly twice daily at the rate of 100ml/ per pot. Because, wheat has an asynchronous fertilization pattern for ovlues in the inflorescence, each floret needs to be specifically marked for timing the fertilization and stress induction. After spikes developed, unfertilized ovules were monitored and observed for the fertilization process. Closed wheat spikes with anthers outside were marked as fertilized. Drought stress was imposed 24h after the fertilization (HAF). Drought stress treatment was initiated by discontinuing watering on the drought treatment plants while control plants were regularly watered twice daily. Stress treatment was applied at 48 HAF and relieved at 96 HAF. The microarray study focuses on 24 HAF to 72 HAF in control and drought stress conditions. We started to impose drought stress 24HAF.

			Action	DRS
	20171024tigernuts.fa	Other
	20171107Nutsedge2nddatabase.fa	Other
	EK_Q_100_1.raw	Raw
	EK_Q_101_1.raw	Raw
	EK_Q_102_1.raw	Raw

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Yellow nutsedge co-opts a seed-like proteome in oil rich tubers

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