Proteomics

Dataset Information

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Plasmodium berghei and Plasmodium falciparum LC-MS/MS

ABSTRACT: Previously published data indicated that PgCHT2 forms a high molecular weight (HMW), reduction-sensitive complex; data suggested that the binding partner is the ookinete-produced von Willebrand A-domain-containing protein, WARP. To test the hypothesis that transgenic P.berghei ookinete-produced PfCHT1 could form a high molecular homo-multimer or, alternatively, could interact with P. berghei ookinete-produced proteins to produce a HMW hetero-multimer, we created a chimeric P. berghei parasites expressing PfCHT1 to replace PbCHT1, allowing production of large numbers of PfCHT1-expressing ookinetes.Mass spectrometry analysis was carried out on the chitin affinity pulled down proteins from the Pb-PfCHT1(r) ookinete culture supernatant.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Plasmodium Berghei Anka

TISSUE(S): Bile

SUBMITTER: Joseph Vinetz  

LAB HEAD: Prof. Jospeh M. Vinetz

PROVIDER: PXD021970 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

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Action DRS
F519268.dat Other
F519268.mgf Mgf
QEp20-0471_Kaur_Chitinase-IP_1.raw Raw
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Publications

A Hetero-Multimeric Chitinase-Containing <i>Plasmodium falciparum</i> and <i>Plasmodium gallinaceum</i> Ookinete-Secreted Protein Complex Involved in Mosquito Midgut Invasion.

Patra Kailash P KP   Kaur Hargobinder H   Kolli Surendra Kumar SK   Wozniak Jacob M JM   Prieto Judith Helena JH   Yates John R JR   Gonzalez David J DJ   Janse Chris J CJ   Vinetz Joseph M JM  

Frontiers in cellular and infection microbiology 20210108

Malaria parasites are transmitted by <i>Anopheles</i> mosquitoes. During its life cycle in the mosquito vector the <i>Plasmodium</i> ookinete escapes the proteolytic milieu of the post-blood meal midgut by traversing the midgut wall. This process requires penetration of the chitin-containing peritrophic matrix lining the midgut epithelium, which depends in part on ookinete-secreted chitinases. <i>Plasmodium falciparum</i> ookinetes have one chitinase (PfCHT1), whereas ookinetes of the avian-infe  ...[more]

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