Dataset Information

Collagen Fiber Regulation in Human Pediatric Aortic Valve Development and Disease

ABSTRACT: Congenital aortic valve stenosis (CAVS) affects up to 10% of the world population without medical therapies to treat the disease. New molecular targets are continually being sought that can halt CAVS progression. Collagen deregulation is a hallmark of CAVS yet remains mostly undefined. Here, histological studies were paired with high resolution accurate mass (HRAM) collagen-targeting proteomics to investigate collagen fiber production with collagen regulation associated with human AV development and pediatric end-stage CAVS (pCAVS). Histological studies identified collagen fiber realignment and unique regions of high-density collagen in pCAVS. Proteomic analysis reported specific collagen peptides are modified by hydroxylated prolines (HYP), a post-translational modification critical to stabilizing the collagen triple helix. Quantitative data analysis reported significant regulation of collagen HYP sites across patient categories. Non-collagen type ECM proteins identified (26/44; 59%) have direct interactions in collagen synthesis, regulation, or modification. Network analysis identified BAMBI (BMP and Activin Membrane Bound Inhibitor) as a potential upstream regulator of the collagen interactome. This is the first study to detail the collagen types and HYP modifications associated with human AV development and pCAVS. We anticipate that this study will inform new therapeutic avenues that inhibit valvular degradation in pCAVS and engineered options for valve replacement.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart, Endothelial Cell, Fibroblast

DISEASE(S): Aortic Valve Insufficiency,Bicuspid Aortic Valve Disease

SUBMITTER: Cassandra Clift

LAB HEAD: Peggi Angel

PROVIDER: PXD021990 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20181221_Peggi_HV_DB007.raw	Raw
	20181221_Peggi_HV_DB032.raw	Raw
	20181221_Peggi_HV_DB051_190206153153.raw	Raw
	20181221_Peggi_HV_DB105.raw	Raw
	20181221_Peggi_HV_DB106.raw	Raw

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Collagen fiber regulation in human pediatric aortic valve development and disease.

Clift Cassandra L CL Su Yan Ru YR Bichell David D Jensen Smith Heather C HC Bethard Jennifer R JR Norris-Caneda Kim K Comte-Walters Susana S Ball Lauren E LE Hollingsworth M A MA Mehta Anand S AS Drake Richard R RR Angel Peggi M PM

Scientific reports 20210507 1

Congenital aortic valve stenosis (CAVS) affects up to 10% of the world population without medical therapies to treat the disease. New molecular targets are continually being sought that can halt CAVS progression. Collagen deregulation is a hallmark of CAVS yet remains mostly undefined. Here, histological studies were paired with high resolution accurate mass (HRAM) collagen-targeting proteomics to investigate collagen fiber production with collagen regulation associated with human AV development ...[more]

PMID: 33963260

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