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Proteomic profile of human stem cells from dental pulp and periodontal ligament

ABSTRACT: Dental stem cells isolated from oral tissues have been shown to provide with high proliferation ability and multi-lineage differentiation potential. Although the dental stem cells possess the potential of nerve regeneration, the specific expression profile of dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) are still unclear. Here, DPSCs and PDLSCs, collected from the same tooth with 3 donors, were tested for high-throughput protein profiles by combining two-dimensional gel proteomics and TMT-based proteomics.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Cell Culture

DISEASE(S): Parkinson's Disease

SUBMITTER: Tong Lei

LAB HEAD: Tong Lei

PROVIDER: PXD021997 | Pride | 2021-06-10

REPOSITORIES: Pride

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Proteomic profile of human stem cells from dental pulp and periodontal ligament.

Lei Tong T Wang Jian J Liu Yanyan Y Chen Peng P Zhang Zhihui Z Zhang Xiaoshuang X Guo Wenhuan W Wang Xiao X Li Quanhai Q Du Hongwu H

Journal of proteomics 20210604

Background The study of molecular profiling of dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) contributes to understanding the high proliferation ability and multi-lineage differentiation potential. Objectives The aim of the study was to compare the protein abundance and specific markers of DPSCs and PDLSCs by protein profiles. Material and methods The DPSCs and PDLSCs extracted from the same tooth were lysed with 3 biological replicates and the protein was collected ...[more]

PMID: 34089896

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Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair [mixed stage]

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Development of gene expression signatures for SOX2 overexpressing human dental pulp stem cells

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