Proteomics

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Comparative Proteomics between Brucella abortus 2308 and Genetically Marked Vaccine A19ΔvirB12 Reveal Attenuation Mechanism and Supplementary Differential Diagnosis Antigens


ABSTRACT: Brucella abortus is an important zoonotic pathogen that causes severe economic loss to husbandry and poses a threat to human health. The B. abortus A19 live vaccine has been extensively used to prevent bovine brucellosis in China. However, A19 has the limitation of diagnosis interference and residual virulence for the host. In this study, a novel genetically marked vaccine, A19ΔvirB12, was generated and evaluated. The results indicated that A19ΔvirB12 was able to provide effective protection against B. abortus 2308 (S2308) challenge in mice and vaccinated sera can be distinguished from infected sera. However, previous studies have found that the accuracy of the serological detection method based on VirB12 protein needs to be improved. Therefore, we attempted to identify potential supplementary antigens with differential diagnosis function using label-free quantitative proteomics. Eighteen proteins identified only in S2308 were screened, and 7 of them were predicted to have high probability of antigenicity. In addition, 12 virulence factors were upregulated in S2308. The upregulation pathways of S2308 were significantly enriched in quorum sensing, ATP-binding cassette transporter, and metabolism. Several proteins related to cell division were significantly downregulated, while some proteins involved in transcription were upregulated in S2308.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Brucella Abortus (strain 2308)

TISSUE(S): Cell Culture

SUBMITTER: Jianghua Yang  

LAB HEAD: zeliang Chen

PROVIDER: PXD022031 | Pride | 2021-09-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Logv01_Fr1.raw Raw
Logv01_Fr2.raw Raw
Logv01_Fr3.raw Raw
Logv01_Fr4.raw Raw
Logv02_Fr1.raw Raw
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Publications

Evaluation and Differential Diagnosis of a Genetic Marked Brucella Vaccine A19ΔvirB12 for Cattle.

Yang Jianghua J   He Chuanyu C   Zhang Huan H   Liu Mengzhi M   Zhao Hailong H   Ren Lisong L   Wu Dongling D   Du Fangyuan F   Liu Baoshan B   Han Xiaohu X   He Sun S   Chen Zeliang Z  

Frontiers in immunology 20210607


<i>Brucella abortus</i> is an important zoonotic pathogen that causes severe economic loss to husbandry and poses a threat to human health. The <i>B. abortus</i> A19 live vaccine has been extensively used to prevent bovine brucellosis in China. However, it is difficult to distinguish the serological response induced by A19 from that induced by natural infection. In this study, a novel genetically marked vaccine, A19ΔvirB12, was generated and evaluated. The results indicated that A19ΔvirB12 was a  ...[more]

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