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Proteomic analysis of NCP's in rat bones

ABSTRACT: The project analyses non-collagenous proteins (NCP's) present in rat bones of various age and sex to determine the various changes that take place in the NCP's with regard to age and sex

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Rattus Norvegicus (rat) Rattus Rattus (black Rat)

SUBMITTER: Manasij Pal Chowdhury

LAB HEAD: Dr. Michael Buckley

PROVIDER: PXD022055 | Pride | 2023-07-24

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	FZ1_F.dat	Other
	FZ1_F.mgf	Mgf
	FZ1_F.raw	Raw
	FZ2_F.dat	Other
	FZ2_F.mgf	Mgf

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Publications

Age-Related Changes in Post-Translational Modifications of Proteins from Whole Male and Female Skeletal Elements.

Johnston Elizabeth E Buckley Michael M

Molecules (Basel, Switzerland) 20230621 13

One of the key questions in forensic cases relates to some form of age inference, whether this is how old a crime scene is, when in time a particular crime was committed, or how old the victim was at the time of the crime. These age-related estimations are currently achieved through morphological methods with varying degrees of accuracy. As a result, biomolecular approaches are considered of great interest, with the relative abundances of several protein markers already recognized for their pote ...[more]

PMID: 37446562

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Proteomic analysis of residues from experimental ceramics

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Proteomic analysis of residues from experimental ceramics

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Proteomic analysis of residues from experimental ceramics used for cooking a mixture of milk, meat and cereals

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Differential gene expression profiles of long bones of telomerase deficient mice (G3-mTERC-/-)

Project description:Telomere shortening due to telomerase deficiency leads to accelerated senescence of human skeletal (mesenchymal) stem cells (MSC) in vitro. In order to study the role of telomere shortening in vivo, we studied the phenotype of telomerase deficient mice caused by absence of telomerase RNA component (TERC-/-). TERC-/- exhibited accelerated age-related bone loss starting at 3 months of age and during 12 months follow up. Bone histomorphometry revealed decreased mineralized surface and bone formation rate as well as increased osteoclast number and size in TERC-/-. Also, serum total deoxy-pyridinoline (tDPD) was increased in TERC-/-. MSC isolated from TERC-/- exhibited intrinsic defects with reduced total number, lower proliferation rate, decreased expression of osteoblastic (OB) differentiation markers and formed less in vivo ectopic bone compared to WT cells. The TERC-/--MSC cultures accumulated a larger proportion of senescent Ã-galactosidase+ cells and cells exhibiting DNA damage positive for Î³-H2AX. Micro-array analysis of bones of TERC-/- and WT revealed significant over-expression of a large number of pro-inflammatory genes and signaling pathways in TERC-/- known to control osteoclast (OC) differentiation. In accordance with that, serum from TERC-/- enhanced OC formation in control bone marrow cultures. Our data demonstrate two mechanisms for age-related bone loss caused by telomerase deficiency: intrinsic osteoblastic defects and creation of pro-inflammatory osteoclast-activating microenvironment. Approaches for re-telomerization of MSC may provide a novel approach for abolishing age-related bone loss. control (WT) and the test (G3 - mTERC-/-) samples

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Tumor-educated platelet extracellular vesicles: proteomic profiling

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A comparison of bone microarchitectural and transcriptomic changes in murine long bones in response to hindlimb unloading and aging

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Deep Proteome profiling reveals signatures of age and sex differences in paw skin and sciatic nerve of naïve mice

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Cross-sectional association of salivary proteins with age, sex, body mass index, smoking, and education

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Serum proteomics in a cohort of COVID patients and healthy controls

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