Proteomics

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Female mating status influences protein composition of mature Drosophila oocytes


ABSTRACT: Oocyte composition can directly influence offspring fitness, particularly in oviparous species such as most insects, where it is the primary form of parental investment. Oocyte production is also energetically costly, dependent on female condition and responsive to external cues. We investigated whether mating influences mature oocyte composition in Drosophila melanogaster using a quantitative proteomic approach. Our analyses robustly identified 4,485 oocyte proteins and revealed that stage-14 oocytes from mated females differed significantly in protein composition relative to oocytes from unmated females. Proteins forming a highly interconnected network enriched for translational machinery and transmembrane proteins were increased in oocytes from mated females, including calcium binding and transport proteins. This mating-induced modulation of oocyte maturation was also significantly associated with proteome changes that are known to be triggered by egg activation. We propose that these compositional changes are likely to have fitness consequences and adaptive implications given the importance of oocyte composition, rather than active gene expression, to the maternal-to-zygotic transition and early embryogenesis.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Oocyte

SUBMITTER: Caitlin McDonough-Goldstein  

LAB HEAD: Steve Dorus

PROVIDER: PXD022142 | Pride | 2021-02-24

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
P473_100619_PeptideGroups.txt Txt
P473_100619_ProteinGroups.txt Txt
P473_100619_Proteins.txt Txt
P473_F1.raw Raw
P473_F10.raw Raw
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Publications

Drosophila oocyte proteome composition covaries with female mating status.

McDonough-Goldstein Caitlin E CE   Pitnick Scott S   Dorus Steve S  

Scientific reports 20210204 1


Oocyte composition can directly influence offspring fitness, particularly in oviparous species such as most insects, where it is the primary form of parental investment. Oocyte production is also energetically costly, dependent on female condition and responsive to external cues. Here, we investigated whether mating influences mature oocyte composition in Drosophila melanogaster using a quantitative proteomic approach. Our analyses robustly identified 4,485 oocyte proteins and revealed that stag  ...[more]

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