Dataset Information

Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms

ABSTRACT: We chracaterized the functions of the understudied LUC7-like family of splicing factors in human cells using seCLIP-seq, RBP knockdown followed by RNA-seq, and Co-IP mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Cell Suspension Culture

DISEASE(S): Acute Leukemia

SUBMITTER: Noah Daniels

LAB HEAD: Richard A. Padgett

PROVIDER: PXD022152 | Pride | 2021-09-09

REPOSITORIES: pride

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Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms.

Daniels Noah J NJ Hershberger Courtney E CE Gu Xiaorong X Schueger Caroline C DiPasquale William M WM Brick Jonathan J Saunthararajah Yogen Y Maciejewski Jaroslaw P JP Padgett Richard A RA

Cell reports 20210401 2

Vertebrates have evolved three paralogs, termed LUC7L, LUC7L2, and LUC7L3, of the essential yeast U1 small nuclear RNA (snRNA)-associated splicing factor Luc7p. We investigated the mechanistic and regulatory functions of these putative splicing factors, of which one (LUC7L2) is mutated or deleted in myeloid neoplasms. Protein interaction data show that all three proteins bind similar core but distinct regulatory splicing factors, probably mediated through their divergent arginine-serine-rich dom ...[more]

PMID: 33852859

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