Dataset Information

Astrocyte conditioned media-dependent Phosphatidylinositol 3-kinase interactome in mouse hippocampal neuron cell line Ht22

ABSTRACT: Astrocytes can support neuronal survival through a range of secreted signals that protect against neurotoxicity, oxidative stress, and apoptotic cascades. To identify proteins contributing to protective intracellular neuronal signalling originating from astrocytes, endogenous PI3K was immunoprecipitated from Ht22 cells exposed to primary astrocyte conditioned media (ACM) or cell free media (CFM), followed by iTRAQ-based quantitative proteomic analysis.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Hippocampal Neuron

DISEASE(S): Wounds And Injuries

SUBMITTER: Declan Williams

LAB HEAD: Gerold Schmitt-Ulms

PROVIDER: PXD022200 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Ht22_p85_interactome_CFM_v_ACM_C18HighpH12_5ACN.raw	Raw
	Ht22_p85_interactome_CFM_v_ACM_C18HighpH17_5ACN.raw	Raw
	Ht22_p85_interactome_CFM_v_ACM_C18HighpH22_5ACN.raw	Raw
	Ht22_p85_interactome_CFM_v_ACM_C18HighpH50_ACN.raw	Raw
	Ht22_p85_interactome_CFM_v_ACM_C18_1-2_HighpH_1-4.msf	Msf

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Publications

An endogenous PI3K interactome promoting astrocyte-mediated neuroprotection identifies a novel association with RNA-binding protein ZC3H14.

Alqawlaq Samih S Livne-Bar Izhar I Williams Declan D D'Ercole Joseph J Leung Sara W SW Chan Darren D Tuccitto Alessandra A Datti Alessandro A Wrana Jeffrey L JL Corbett Anita H AH Schmitt-Ulms Gerold G Sivak Jeremy M JM

The Journal of biological chemistry 20201203

Astrocytes can support neuronal survival through a range of secreted signals that protect against neurotoxicity, oxidative stress, and apoptotic cascades. Thus, analyzing the effects of the astrocyte secretome may provide valuable insight into these neuroprotective mechanisms. Previously, we characterized a potent neuroprotective activity mediated by retinal astrocyte conditioned media (ACM) on retinal and cortical neurons in metabolic stress models. However, the molecular mechanism underlying t ...[more]

PMID: 33234594

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