Dataset Information

Proteomics of aqueous humor for biomarkers discovery in retinoblastoma

ABSTRACT: Background: Recent therapeutic advances have greatly improved the eye preservation rate in patients affected by retinoblastoma (RB), however local tumor control remains more difficult in the presence of resistant and/or relapsing retinal or vitreous disease. Thus, the identification of new biomarkers is crucial to design more effective treatment approaches. Recently, technological advances have allowed to safely recover aqueous humor (AH) from patients affected by RB undergoing local treatment. Methods: in order to identify specific proteins associated to active vitreous seeding, we have performed proteomic analysis of AH samples derived from patients affected by RB in different disease stages. Fifty-five AH samples were firstly included in this study. A new widened cohort was then used to validate the proteins identified in the previous discovery set. Results: a total of 808 and 630 proteins were identified in the two analysis, respectively. Five proteins resulted more expressed in the active vitreous seeding group: C19orf57, Girdin, SRFBP, GULP-1 and DCD. These proteins are involved in cytoskeleton regulation, tumor proliferation and growth, invasion and migration of cancer cells. Conclusion: reliable prognostic biomarkers are needed to guide RB management. Proteomic analysis of AH provide a unique opportunity to identify tumor associated biomarkers in patients with RB.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Vitreous Humor

DISEASE(S): Retinoblastoma

SUBMITTER: Andrea Petretto

LAB HEAD: Andrea Petretto

PROVIDER: PXD022218 | Pride | 2023-03-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Active_Vitreal_Seeding_119_063.raw	Raw
	Active_Vitreal_Seeding_126_064.raw	Raw
	Active_Vitreal_Seeding_A4_043.raw	Raw
	Active_Vitreal_Seeding_A5_056.raw	Raw
	Active_Vitreal_Seeding_B1_060.raw	Raw

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Publications

Proteomics of Aqueous Humor as a Source of Disease Biomarkers in Retinoblastoma.

Galardi Angela A Stathopoulos Christina C Colletti Marta M Lavarello Chiara C Russo Ida I Cozza Raffaele R Romanzo Antonino A Carcaboso Angel M AM Locatelli Franco F Petretto Andrea A Munier Francis L FL Di Giannatale Angela A

International journal of molecular sciences 20221103 21

Aqueous humor (AH) can be easily and safely used to evaluate disease-specific biomarkers in ocular disease. The aim of this study was to identify specific proteins biomarkers in the AH of retinoblastoma (RB) patients at various stages of the disease. We analyzed the proteome of 53 AH samples using high-resolution mass spectrometry. We grouped the samples according to active vitreous seeding (Group 1), active aqueous seeding (Group 2), naive RB (group 3), inactive RB (group 4), and congenital cat ...[more]

PMID: 36362243

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