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Circadian Profiling of the Bone Marrow Derived Macrophage Proteome

ABSTRACT: Triplicate circadian time courses in synchronized macrophages sampling every 2 hours over 48 hours, starting at 16 hours post serum shock. Global proteome at each time point was quantified with 8 TMT10plex experiments.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Bone Marrow, Macrophage

SUBMITTER: Emily Collins

LAB HEAD: Jennifer Hurley

PROVIDER: PXD022260 | Pride | 2021-02-12

REPOSITORIES: Pride

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	070717_tc-d436_443_protquant.xlsx	Xlsx
	7879_c03295_tc-d436_F1A.mzIdentML	Mzid
	7880_c03296_tc-d436_F2A.mzidentml	Mzid
	7881_c03297_tc-d436_F3A.mzidentml	Mzid
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Publications

Post-transcriptional circadian regulation in macrophages organizes temporally distinct immunometabolic states.

Collins Emily J EJ Cervantes-Silva Mariana P MP Timmons George A GA O'Siorain James R JR Curtis Annie M AM Hurley Jennifer M JM

Genome research 20210112 2

Our core timekeeping mechanism, the circadian clock, plays a vital role in immunity. Although the mechanics of circadian control over the immune response is generally explained by transcriptional activation or repression derived from this clock's transcription-translation negative-feedback loop, research suggests that some regulation occurs beyond transcriptional activity. We comprehensively profiled the transcriptome and proteome of murine bone marrow-derived macrophages and found that only 15% ...[more]

PMID: 33436377

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