Proteomics

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Multiplexed CuAAC-Suzuki-Miyaura for tandem activity-based chemoproteomic profiling

ABSTRACT: Suzuki-Miyauara cross-coupling has been used for mass-spectrometry-based chemoproteomics, including for target deconvolution and site of labeling studies. We also demonstrated that Suzuki-Miyauara cross coupling and CuAAC can be combined for dual target labeling for mass spectrometry-based proteomics. Furthermore, multiplexed CuAAC Suzuki-Miyaura chemoproteomics platform has been successfully applied to identify the protein targets of bifunctional cysteine- and lysine-reactive crosslinking probes.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Keriann Backus  

LAB HEAD: Keriann Backus

PROVIDER: PXD022279 | Pride | 2021-02-03

REPOSITORIES: Pride

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Publications

Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling.

Cao Jian J   Boatner Lisa M LM   Desai Heta S HS   Burton Nikolas R NR   Armenta Ernest E   Chan Neil J NJ   Castellón José O JO   Backus Keriann M KM  

Analytical chemistry 20210120 4

Mass-spectrometry-based chemoproteomics has enabled the rapid and proteome-wide discovery of functional and potentially 'druggable' hotspots in proteins. While numerous transformations are now available, chemoproteomic studies still rely overwhelmingly on copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or 'click' chemistry. The absence of bio-orthogonal chemistries that are functionally equivalent and complementary to CuAAC for chemoproteomic applications has hindered the development of m  ...[more]

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