Dataset Information

Confirmation of plant viral proteins and identification of specific viral strains by nanoLC-ESI-Q-TOF using single-leaf-tissue samples

ABSTRACT: An efficient protein extraction protocol without any precipitation steps was applied to plant samples from ten economically important plant hosts. Viral proteins from fourteen important viruses (WDV, BYDV/BYDV-PAV, BMV, TAV, CaMV, TMV, TVCV, PPV, TuMV, BCMV, SrMV, SCMV, TRSV, BBWV-2) from seven different families (Geminiviridae, Luteoviridae, Bromoviridae, Caulimoviridae, Virgaviridae, Potyviridae, Secoviridae) were detected in infected samples. Protein database of host proteins and potential pathogen proteins was assembled separately for each host and based on existing online plant virus pathogen lists. For PPV, WDV, BYDV a succesfull discrimination to virus strains (as demonstrated for PPV, WDV) or distinct disease species (BYDV) was also demonstrated.

INSTRUMENT(S): maXis

ORGANISM(S): Nicotiana Tabacum (common Tobacco) Prunus Armeniaca Chenopodium Giganteum Brassica Rapa Subsp. Pekinensis Hordeum Vulgare (barley) Phaseolus Vulgaris (kidney Bean) (french Bean) Prunus Domestica Sorghum Bicolor Zea Mays (maize) Triticum Aestivum (wheat)

TISSUE(S): Plant Cell, Leaf

DISEASE(S): Viral Infectious Disease

SUBMITTER: Pavel Cejnar

LAB HEAD: Pavel Cejnar

PROVIDER: PXD022456 | Pride | 2020-11-20

REPOSITORIES: Pride

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Efficient Confirmation of Plant Viral Proteins and Identification of Specific Viral Strains by nanoLC-ESI-Q-TOF Using Single-Leaf-Tissue Samples.

Cejnar Pavel P Kučková Štěpánka Š Šantrůček Jiří J Glasa Miroslav M Komínek Petr P Mihálik Daniel D Slavíková Lucie L Leišová-Svobodová Leona L Smirnova Tatiana T Hynek Radovan R Kundu Jiban Kumar JK Ryšánek Pavel P

Pathogens (Basel, Switzerland) 20201119 11

Plant viruses are important pathogens that cause significant crop losses. A plant protein extraction protocol that combines crushing the tissue by a pestle in liquid nitrogen with subsequent crushing by a roller-ball crusher in urea solution, followed by RuBisCO depletion, reduction, alkylation, protein digestion, and ZipTip purification allowed us to substantially simplify the sample preparation by removing any other precipitation steps and to detect viral proteins from samples, even with less ...[more]

PMID: 33228257

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