Dataset Information

Proteomic analyses of lysine 2-hydroxyisobutyrylation in Fusarium graminearum in response to tebuconazole and carbendazim

ABSTRACT: Lysine 2-hydroxyisobutyrylation (Khib) is a newly discovered post-translational modification in some prokaryotic and eukaryotic organisms. Here, we carried out proteome-wide analysis of Khib in Fusarium graminearum, identifying the reshaping of lysine 2-hydroxyisobutyrylome by tebuconazole or carbendazim, using the most recently-developed high-resolution LC-MS/MS in combination with high-specific affinity enrichment. In total, 3035 quantifiable Khib sites on 937 proteins were identified. With the criteria of fold changes over 1.3, normalized with total proteins, 1083 Khib sites of 509 proteins were considered as significantly affected by tebuconazole treatment, while 344 Khib sites of 227 proteins were significantly affacted by carbendazim. Furthermore, bioinformatics analysis were conducted.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Gibberella Zeae (strain Ph-1 / Atcc Mya-4620 / Fgsc 9075 / Nrrl 31084) (wheat Head Blight Fungus) (fusarium Graminearum)

SUBMITTER: Yanxiang Zhao

LAB HEAD: Jinguang Huang

PROVIDER: PXD022499 | Pride | 2022-04-04

REPOSITORIES: Pride

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			Action	DRS
	F9111TP2o.raw	Raw
	mqpar.xml	Xml
	search.zip	Other

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Publications

Quantitative multiplexed proteomics analysis reveals reshaping of the lysine 2-hydroxyisobutyrylome in Fusarium graminearum by tebuconazole.

Zhao Yanxiang Y Zhang Limin L Ju Chao C Zhang Xiaoyan X Huang Jinguang J

BMC genomics 20220218 1

<h4>Backgrounds</h4>Lysine 2-hydroxyisobutyrylation (Khib) is a newly discovered posttranslational modification (PTM) and has been identified in several prokaryotic and eukaryotic organisms. Fusarium graminearum, a major pathogen of Fusarium head blight (FHB) in cereal crops, can cause considerable yield loss and produce various mycotoxins that threaten human health. The application of chemical fungicides such as tebuconazole (TEC) remains the major method to control this pathogen. However, the ...[more]

PMID: 35180840

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