Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Fasciola hepatica egg lysates - investigation of proteolytic activity

ABSTRACT: In order to investigate proteolytic activity (presence of peptidases) in eggs of F. hepatica, we performed LC-MS analysis of eggs. Eggs were colelcted fresh and then after 5 and 10 days of incubation (embryonation). Each time point done in triplicates.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Fasciola Hepatica (liver Fluke)

TISSUE(S): Egg, Oocyte

DISEASE(S): Fascioliasis

SUBMITTER: Pavel Roudnický

LAB HEAD: Zbyněk Zdráhal

PROVIDER: PXD022516 | Pride | 2022-07-11

REPOSITORIES: pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	3885_Kasny_150min-IS_HCD-OT_T0_1.raw	Raw
	3885_Kasny_150min-IS_HCD-OT_T0_2.raw	Raw
	3885_Kasny_150min-IS_HCD-OT_T0_3.raw	Raw
	3885_Kasny_150min-IS_HCD-OT_T10_1.raw	Raw
	3885_Kasny_150min-IS_HCD-OT_T10_2.raw	Raw

Items per page:

1 - 5 of 11

Similar Datasets

Linking planar polarity signaling directly to actomyosin contractility during vertebrate neurulation

Project description:Actomyosin contractility represents an ancient feature of eukaryotic cells participating in many developmental and homeostasis events, including tissue morphogenesis, muscle contraction, and cell migration, with dysregulation implicated in various pathological conditions, such as cancer. At the molecular level, actomyosin comprises actin bundles and myosin motor proteins that are sensitive to posttranslational modifications like phosphorylation. While the molecular components of actomyosin and its regulation are well understood, the coordination of contractility by extracellular and intracellular signals, particularly from cellular signaling pathways, remains incompletely elucidated. This study focuses on planar cell polarity (PCP) signaling, previously associated with actomyosin contractility during vertebrate neurulation. Contrary to previous studies, our investigation reveals that main cytoplasmic PCP proteins, Prickle and Dishevelled, interact directly with key actomyosin components such as myosin light chain (MLC), leading to its phosphorylation and localized activation. Through relevant in vitro and in vivo models, we demonstrate that these PCP proteins function not merely passively and indirectly as previously reported, but that they actively control actomyosin contractility by acting as scaffold proteins, orchestrating the necessary machinery for contractility. These findings unveil a direct link of PCP signaling in crucial actomyosin contractility processes through MLC that are relevant to vertebrate neurulation and potentially beyond.

2024-09-19 | PXD052350 | Pride

Proteomic analysis of drought stress response in Opium poppy

Project description:Main aim of the project is to analyze differentially expressed genes and proteins in early germination stages of Opium poppy plants under drought stress conditions. These results will be used for selection of drought resistant varieties for dry regions and also for further breeding of drought resistant varieties. Side aim of the project is to utilize standard set of methods for sample preparation and processing for proteomic and transcriptomic analysis, that would lead to more comparable results for future assessments in the field of poppy drought stress resistance research.

2022-02-17 | PXD027435 | Pride

Protein composition in thylakoids of Arabidopsis without Lhcb3 and Lhcb6

Project description:The aim of the project is to estimate the relative protein content of photosynthetic proteins in thylakoids of Arabidopsis plants without antenna proteins Lhcb6 and Lhcb3. 4 sample types: WT, koLhcb3, koLhcb6, koLhcb3+6

2021-08-09 | PXD023071 | Pride

Identification of proteins in separated PSII supercomplexes from spruce (P. abies)

Project description:To identify protein composition of photosystem II (PSII) supercomplexes separated from spruce seedlings using sucrose gradient centrifugation. These supercomplexes represent mainly so called C2S2 PSII supercomplex as revealed by high-resolution cryo-EM.

2023-07-19 | PXD035272 | Pride

Protein composition of photosystem II supercomplexes from Arabidopsis

Project description:The goal of the project is to identify the protein composition (and their relative abundance) of selected photosystem II supercomplexes separated using mild CN-PAGE from thylakoid membranes of Arabidopsis plants lacking proteins i) Lhcb6, ii) Lhcb3, iii) Lhcb3 and Lhcb6. These proteomic data will be compared with single-particle EM data obtained in the separated photosystem II supercomplexes.

2021-08-07 | PXD026019 | Pride

Arabidopsis and spruce grown at different light intensity - proteins in thylakoids

Project description:The aim of the project is to estimate the relative protein content of photosynthetic proteins in thylakoids isolated from Arabidopsis leaves and spruce needles grown at different light intensity (high, normal, low).

2022-08-18 | PXD029868 | Pride

Long-term zinc-treated human prostate cancer cells, LC-MS

Project description:Failure in intracellular zinc accumulation is a key process in prostate cancerogenesis. Although prostate cancer cells can accumulate zinc after long-term exposure, chronic zinc oversupply may accelerate prostate carcinogenesis or chemoresistance. As a model of prostate cancerogenesis, long-term zinc-treated (zinc accumulating) cell lines 22Rv1, and PC-3 were used. In this dataset we investigated how long-term zinc treatment is associated with changes of cell proteome in these cells as determined by LC-MS. Preparation of long-term zinc-treated cells is described in Holubova et al., 2014, and Raudenska et al, 2019.

2024-09-11 | PXD046410 | Pride

The beta subunit of nascent polypeptide associated complex plays a role in flowers and siliques development of Arabidopsis thaliana

Project description:The nascent polypeptide-associated (NAC) complex was described in yeast as a heterodimer composed of two subunits, α and β, and was shown to bind to the nascent polypeptides newly emerging from the ribosome. Although NAC function was widely described in yeast, less is known about its role in plants. The knock down of individual NAC subunit(s) led usually to a higher sensitivity to stress. In Arabidopsis thaliana genome, there are five genes coding for NACα subunit, and two genes coding for NACβ. Double homozygous mutant in both genes coding for NACβ was acquired, which showed a delayed development compared to the wild type, had abnormal number of flower organs, shorter siliques and greatly reduced seed set. Herein, both NACβ genes were characterized by complementation analysis, overexpression, subcellular localization, and promoter analysis. Since flowers were the most affected organs by nacβ mutation, the flower buds transcriptome was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NACβ subunits in stress responses and male gametophyte development.

2020-05-27 | PXD016315 | Pride

Arabidopsis thaliana peptide SP2 and ethyl acetate extraction clean up.

Project description:The success of bottom-up proteomic analysis frequently depends on the efficient removal of contaminants from protein or peptide samples before LC-MS/MS. For a peptide clean-up workflow, the single-pot solid-phase-enhanced peptide sample preparation on carboxylate-modified paramagnetic beads (termed SP2) was evaluated for sodium dodecyl sulfate or polyethylene glycol removal from Arabidopsis thaliana tryptic peptides. The robust and efficient 40-min SP2 protocol, tested for a 10 ng, 250ng and 10µg peptide sample, was proposed and benchmarked thoroughly against the ethyl acetate extraction protocol. The SP2 protocol on carboxylated magnetic beads proved to be the robust approach even for simultaneous removal of massive sodium dodecyl sulfate (SDS) and polyethylene glycol (PEG) contaminations from AT peptide samples in respect of the LC-MS/MS data outperforming ethyl acetate extraction.

2024-01-03 | PXD045031 | Pride

Staphylococcus aureus prophage-encoded protein causes abortive infection and provides population immunity against kayviruses

Project description:The aim of the study was to investigate the resistance mechanism of Staphylococcus aureus towards lytic phages of the genus Kayvirus and the role of the membrane-anchored protein (primary accession Q2FYE0) designated PdpSau encoded by Staphylococcus aureus prophages. PdpSau does not prevent the infecting kayvirus from adsorbing onto the host cell and delivering its genome into the cell, but phage DNA replication is halted. Changes in the cell membrane polarity and permeability were observed 10 min after the infection leading to prophage-activated cell death. The LC-MS/MS analysis, as one of the methods, was used for protein detection and to find out whether this protein is predominantly presented in membranes. These findings are relevant for the advancement of phage therapy.

2023-02-03 | PXD036676 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data