Proteomics

Dataset Information

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Biomarkers of pediatric cataracts: a proteomics analysis of aqueous fluid


ABSTRACT: Abstract Background: Cataracts are among the most common causes of childhood vision loss worldwide. This study seeks to identify differentially expressed proteins in the aqueous humor of pediatric cataract patients. Methods: Samples of aqueous humor were collected from pediatric and adult cataract patients and underwent mass spectrometry-based proteomic analysis. Samples of pediatric cataracts were grouped by subtype and compared to adult samples. Differentially expressed proteins in each subtype were identified. Gene ontology analysis was performed using WikiPaths for each cataract subtype. Results: Seven pediatric patients and ten adult patients were included in the study. Of the pediatric samples, all seven (100%) were male, three (43%) had traumatic cataracts, two (29%) had congenital cataracts, and two (29%) had posterior polar cataracts. Of the adult patients, seven (70%) were female and seven (70%) had predominantly nuclear sclerotic cataracts. 128 proteins were upregulated in the pediatric samples and 127 proteins were upregulated in the adult samples, with 75 proteins shared by both groups. Gene ontology analysis identified inflammatory and oxidative stress pathways as upregulated in pediatric cataracts. Conclusions: Inflammatory and oxidative stress mechanisms may be involved in pediatric cataract formation and warrant further investigation.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Vitreous Humor

DISEASE(S): Wounds And Injuries

SUBMITTER: Donald Wolfgeher  

LAB HEAD: Dr. Sarah Hilkert Rodrigue, MD, MPH

PROVIDER: PXD022529 | Pride | 2023-07-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
200420_AqH_Project_SampleIndex.xlsx Xlsx
200501_AqH_MQ_Index.txt Txt
200501_SPROT_Human_UP5640.fasta Fasta
200518_AqH_1FDR.sps Other
200518_AqH_MQ1FDR_Final.xlsx Xlsx
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Publications

Biomarkers of Pediatric Cataracts: A Proteomics Analysis of Aqueous Fluid.

Theophanous Christos N CN   Wolfgeher Donald J DJ   Farooq Asim V AV   Hilkert Rodriguez Sarah S  

International journal of molecular sciences 20230520 10


Cataracts are among the most common causes of childhood vision loss worldwide. This study seeks to identify differentially expressed proteins in the aqueous humor of pediatric cataract patients. Samples of aqueous humor were collected from pediatric and adult cataract patients and subjected to mass spectrometry-based proteomic analysis. Samples of pediatric cataracts were grouped by subtype and compared to adult samples. Differentially expressed proteins in each subtype were identified. Gene ont  ...[more]

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