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Proteomic analysis reveals critical immunomolecular mechanisms in macrophage anti-tuberculosis processes

ABSTRACT: Tuberculosis infection activates the autoimmune system. However, the role of autoimmunity after Mycobacterium tuberculosis infection is unclear. In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with tandem mass spectrometry (TMT) was used to perform proteomic analysis of five spinal tuberculosis and five protruding disc tissue samples. The identification of key molecular mechanisms provides a molecular target for future treatment of spinal tuberculosis, which may significantly improve patients' quality of life and prognosis.

INSTRUMENT(S): 6410 Triple Quadrupole LC/MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Intervertebral Disc

SUBMITTER: Xinli Zhan

LAB HEAD: Xinli Zhan

PROVIDER: PXD022541 | Pride | 2021-01-04

REPOSITORIES: Pride

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Proteomic analysis reveals critical molecular mechanisms involved in the macrophage anti-spinal tuberculosis process.

Xu Guoyong G Xue Jiang J Jiang Jie J Liang Tuo T Yao Yuanlin Y Liao Shian S Chen Tianyou T Li Hao H Liu Chong C Zhan Xinli X

Tuberculosis (Edinburgh, Scotland) 20201209

Tuberculosis infection activates the autoimmune system. However, the role of host-pathogen interactions involved in Mycobacterium tuberculosis infection is unclear. In this study, we analyzed 6 spinal tuberculosis tissues and 6 herniated disc tissues by using liquid chromatography-tandem mass spectrometry coupled with tandem mass spectrometry, and immunohistochemical staining was performed for validating the results. We identified 42 differential immune-related proteins and 3 hub genes that are ...[more]

PMID: 33316736

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