Proteomics

Dataset Information

0

Mass spectrometry data for Leishmania major proteins obtained by immunoprecipitation with anti-PUF1, anti-PUF3 or pre-immunization antibodies.


ABSTRACT: This project was aimed to the characterization of proteins interacting with the RNA binding proteins PUF1 and PUF3 of Leishmania major (Friedlin strain). For this purpose an immunoprecipitation (IP) strategy was followed. Firstly, protein extracts from L. major promastigotes were incubated with specific antibodies, raised in rabbits, against recombinant proteins PUF1 or PUF3. In parallel, a control IP using a pre-immune serum was performed. After affinity purification of the antibody-protein complexes, proteins were analyzed by Reverse phase- Liquid Chomatography/Mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Leishmania Major

SUBMITTER: Jose María Requena  

LAB HEAD: Jose M. Requena

PROVIDER: PXD022581 | Pride | 2021-05-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
140416_Irene_1.mgf Mgf
140416_Irene_1.mzid.gz Mzid
140416_Irene_1.raw Raw
140416_Irene_2.mgf Mgf
140416_Irene_2.mzid.gz Mzid
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