Revealing Candida glabrata biofilm matrix proteome: global characterization and pH response

ABSTRACT: Candida glabrata is a clinically relevant human pathogen with ability to form high recalcitrant biofilms that contribute to the establishment and persistence of infection. A defining trait of biofilms is the auto-produced matrix, which is suggested to have structural, virulent and protective roles. Thus, elucidation of matrix components, their function and modulation by host environment is crucial to disclose their role in C. glabrata pathogenesis. As a major step toward this end, this study aimed to reveal, for the first time, the matrix proteome of C. glabrata biofilms, to characterize it with bioinformatic tools and to study its modulation by the environmental pH (acidic and neutral). The results showed the presence of several pH-specific matrix proteins (51 acidic- and 206 neutral-specific) and also proteins commonly found at both pH conditions (236). Of note, several proteins related to β-glucan metabolism, which have a potential role in the delivery/organization of matrix glucans, were found in both conditions but in much higher quantity under the neutral environment. Additionally, proteins with roles in adhesion, including epithelial adhesins, and virulence-related, including yapsins, were found among pH-specific and non-specific matrix proteins. Importantly, several proteins seem to have a non-canonical secretion pathway and Pdr1 was found to be a potential regulator of matrix proteome. Overall, this study indicates a relevant impact of environmental cues in the matrix proteome and provides a unique resource for further functional investigation of matrix proteins, contributing to the identification of potential targets for the development of new therapies against C. glabrata biofilms.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Candida Glabrata (yeast) (torulopsis Glabrata)

SUBMITTER: Hugo Osorio  

LAB HEAD: Hugo Osório

PROVIDER: PXD022597 | Pride | 2024-02-22

REPOSITORIES: Pride