A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI-SNF complex component

ABSTRACT: Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have assessed the specificity of a polyclonal antibody (Bethyl A302-276A) that is commonly used as a probe for NCAPH2, the kleisin subunit of condensin II, in mammalian cells. We find that, in addition to its intended target, this antibody cross-reacts with one or more components of the SWI-SNF family of chromatin remodelling complexes in an NCAPH2-independent manner.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: James Wright  

LAB HEAD: Jyoti Choudhary

PROVIDER: PXD022620 | Pride | 2020-12-14

REPOSITORIES: Pride