Proteomics

Dataset Information

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Metaproteomic analyses of microbial community from ancient permafrost


ABSTRACT: It remains challenging to study the viability and metabolic activity of microorganisms buried in ancient permafrost. We coupled aspartic acid racemization assay with metaproteomics to constraint the microbial activity of indigenous microbial community entrappped in permafrost sediment over 100 kyr ago.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Permafrost Metagenome

SUBMITTER: Renxing Liang  

LAB HEAD: Tullis Onstott

PROVIDER: PXD022683 | Pride | 2021-09-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
RX_Depth14dot8_Run1.pep.txt Txt
RX_Depth14dot8_Run1_01.raw Raw
RX_Depth14dot8_Run1_02.raw Raw
RX_Depth14dot8_Run1_03.raw Raw
RX_Depth14dot8_Run1_04.raw Raw
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Publications


<h4>Background</h4>Total DNA (intracellular, iDNA and extracellular, eDNA) from ancient permafrost records the mixed genetic repository of the past and present microbial populations through geological time. Given the exceptional preservation of eDNA under perennial frozen conditions, typical metagenomic sequencing of total DNA precludes the discrimination between fossil and living microorganisms in ancient cryogenic environments. DNA repair protocols were combined with high throughput sequencing  ...[more]

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