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Metaproteomic analyses of microbial community from ancient permafrost

ABSTRACT: It remains challenging to study the viability and metabolic activity of microorganisms buried in ancient permafrost. We coupled aspartic acid racemization assay with metaproteomics to constraint the microbial activity of indigenous microbial community entrappped in permafrost sediment over 100 kyr ago.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Permafrost Metagenome

SUBMITTER: Renxing Liang

LAB HEAD: Tullis Onstott

PROVIDER: PXD022683 | Pride | 2021-09-09

REPOSITORIES: Pride

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			Action	DRS
	RX_Depth14dot8_Run1.pep.txt	Txt
	RX_Depth14dot8_Run1_01.raw	Raw
	RX_Depth14dot8_Run1_02.raw	Raw
	RX_Depth14dot8_Run1_03.raw	Raw
	RX_Depth14dot8_Run1_04.raw	Raw

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Publications

Genomic reconstruction of fossil and living microorganisms in ancient Siberian permafrost.

Liang Renxing R Li Zhou Z Lau Vetter Maggie C Y MCY Vishnivetskaya Tatiana A TA Zanina Oksana G OG Lloyd Karen G KG Pfiffner Susan M SM Rivkina Elizaveta M EM Wang Wei W Wiggins Jessica J Miller Jennifer J Hettich Robert L RL Onstott Tullis C TC

Microbiome 20210517 1

<h4>Background</h4>Total DNA (intracellular, iDNA and extracellular, eDNA) from ancient permafrost records the mixed genetic repository of the past and present microbial populations through geological time. Given the exceptional preservation of eDNA under perennial frozen conditions, typical metagenomic sequencing of total DNA precludes the discrimination between fossil and living microorganisms in ancient cryogenic environments. DNA repair protocols were combined with high throughput sequencing ...[more]

PMID: 34001281

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