Proteomics

Dataset Information

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Integrative analysis of two russeted and non-russeted closely related apple varieties during fruit development provides new insights about the cuticle/suberin interplay


ABSTRACT: Apple russeting is characterized by the deposition of suberin in the inner part of the epidermal cell walls. It develops at the fruit surface when the integrity of the cuticle has been lost. Cutin and wax composition (including triterpene profile) is also affected in russeted skin. In the present work, the fruit growth kinetic of two closely related apple varieties, ‘Canada Gris’ and ‘Canada Blanc’, which display russeted and waxy skin phenotypes, respectively, was investigated. We used a combination of transcriptomic, proteomic, and metabolomic approaches to better understand the molecular events associated with cuticle failure and suberization. A particular interest was also given to the triterpene specifically found in russeted skins and their synthesis. Our results indicate that the decreased synthesis of cutin and wax, observed in russeted skin throughout fruit development, is a determining factor for russeting. We identified a lipid transfer protein (LTP-3) as a potential key player in cuticle formation. Metabolomics highlighted a large diversity of triterpene-hydroxycinnamates in russeted tissues whose accumulation was highly correlated with suberin-related genes, including some enzymes belonging to the BAHD (HXXXD-motif) acyltransferase family. This study shed light for the first time on the crosstalk between triterpene and suberin pathways.

INSTRUMENT(S): TripleTOF 5600+

ORGANISM(S): Malus Domestica

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Céline LECLERCQ  

LAB HEAD: Céline LECLERCQ

PROVIDER: PXD022712 | Pride | 2022-02-22

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
2010Mdomestica_196_v1.0.proteinannot.fasta Fasta
F122324.dat Other
F122324.mzid Mzid
F122324.xml Xml
F122325.dat Other
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Publications

Identification of Novel Candidate Genes Involved in Apple Cuticle Integrity and Russeting-Associated Triterpene Synthesis Using Metabolomic, Proteomic, and Transcriptomic Data.

André Christelle M CM   Guerriero Gea G   Lateur Marc M   Charton Sophie S   Leclercq Celine C CC   Renaut Jenny J   Hausman Jean-Francois JF   Legay Sylvain S  

Plants (Basel, Switzerland) 20220121 3


Apple russeting develops on the fruit surface when skin integrity has been lost. It induces a modification of fruit wax composition, including its triterpene profile. In the present work, we studied two closely related apple varieties, 'Reinette grise du Canada' and 'Reinette blanche du Canada', which display russeted and non-russeted skin phenotypes, respectively, during fruit development. To better understand the molecular events associated with russeting and the differential triterpene compos  ...[more]

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