Proteomics

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Categorization of Phosphorylation Site Behavior During the Diauxic Shift in Saccharomyces cerevisiae


ABSTRACT: Protein phosphorylation has long been recognized as an essential regulator of protein activity, structure, complex formation, and sub-cellular localization among other cellular mechanisms. However, interpretation of the changes in protein phosphorylation is difficult. To address this difficulty, we measured protein and phosphorylation site changes across 11 points of a time course and developed a method for categorizing phosphorylation site behavior relative to protein level changes using the diauxic shift in yeast as a model and TMT11 sample multiplexing. We classified quantified proteins into behavioral categories that reflected differences in kinase activity, protein complex structure, and growth and metabolic pathway regulation across different phases of the diauxic shift. These data also provide a valuable resource for the study of fermentative versus respiratory growth and set a new benchmark for temporal quantitative proteomics and phosphoproteomics for Diauxic Shift in Saccharomyces cerevisiae.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Brandon Gassaway  

LAB HEAD: Steven Gygi

PROVIDER: PXD022741 | Pride | 2021-03-04

REPOSITORIES: Pride

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Categorization of Phosphorylation Site Behavior during the Diauxic Shift in <i>Saccharomyces cerevisiae</i>.

Gassaway Brandon M BM   Paulo Joao A JA   Gygi Steven P SP  

Journal of proteome research 20210225 5


Protein phosphorylation has long been recognized as an essential regulator of protein activity, structure, complex formation, and subcellular localization among other cellular mechanisms. However, interpretation of the changes in protein phosphorylation is difficult. To address this difficulty, we measured protein and phosphorylation site changes across 11 points of a time course and developed a method for categorizing phosphorylation site behavior relative to protein level changes using the dia  ...[more]

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