Proteomics

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Budding yeast relies on qualitative G1 cyclin specificity to couple cell cycle progression with morphogenetic development

ABSTRACT: Two models have been put forward for cyclin-dependent kinase (Cdk) control of the cell cycle. In the qualitative model, cell cycle events are ordered by distinct substrate specificities associated with successive waves of G1, S and mitotic cyclins. Alternatively, the gradual quantitative rise of Cdk activity from G1 phase to mitosis could lead to ordered substrate phosphorylation at sequential thresholds. Here, we study the relative contributions of qualitative and quantitative Cdk control in the budding yeast S. cerevisiae. S-phase cyclins can be replaced by a single mitotic cyclin, albeit at the cost of reduced fitness. The single cyclin can in addition replace G1 cyclins to support ordered cell cycle progression, fulfilling key predictions of the quantitative model. However, single-cyclin cells fail to polarize or grow buds and thus cannot sustain proliferation. Our results suggest that budding yeast has become dependent on G1 cyclin specificity to couple cell cycle progression to essential morphogenetic events.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Helen Flynn  

LAB HEAD: Frank Uhlmann

PROVIDER: PXD022757 | Pride | 2021-08-23

REPOSITORIES: Pride

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Budding yeast relies on G<sub>1</sub> cyclin specificity to couple cell cycle progression with morphogenetic development.

Pirincci Ercan Deniz D   Chrétien Florine F   Chakravarty Probir P   Flynn Helen R HR   Snijders Ambrosius P AP   Uhlmann Frank F  

Science advances 20210604 23

Two models have been put forward for cyclin-dependent kinase (Cdk) control of the cell cycle. In the qualitative model, cell cycle events are ordered by distinct substrate specificities of successive cyclin waves. Alternatively, in the quantitative model, the gradual rise of Cdk activity from G<sub>1</sub> phase to mitosis leads to ordered substrate phosphorylation at sequential thresholds. Here, we study the relative contributions of qualitative and quantitative Cdk control in <i>Saccharomyces  ...[more]

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