Dataset Information

Comparative proteomics for identifying specific alterations within interferon response pathways in human glioblastoma multiforme and osteosarcoma cells

ABSTRACT: An acquisition of increased sensitivity of cancer cells to viruses is a common outcome of malignant progression that justifies the development of oncolytic viruses as anticancer therapeutics. Studying molecular changes that underlie the sensitivity to viruses would help to identify cases where oncolytic virus therapy would be most effective. We quantified changes in protein abundances in glioblastoma multiforme (GBM) and osteosarcoma cell lines that differ in the ability to induce resistance to vesicular stomatitis virus (VSV) infection in response to type I interferon (IFN) treatment. In IFN-treated samples we observed an up-regulation of protein products of some IFN-regulated genes (IRGs). In total, the proteome analysis revealed up to 20% more proteins encoded by IRGs in the glioblastoma cell line, which develops resistance to VSV infection after pre-treatment with IFN. In both cell lines protein-protein interaction and signaling pathway analyses have revealed a significant stimulation of processes related to type I IFN signaling and defense responses to viruses. The study has shown that the up-regulation of IRG proteins induced by the IFNα treatment of GBM cells can be detected at the proteome level. Similar analyses could be applied for revealing functional alterations within the antiviral mechanisms in glioblastoma samples, accompanying by acquisition of sensitivity to oncolytic viruses.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, Permanent Cell Line Cell

DISEASE(S): Glioblastoma

SUBMITTER: Victoria Lineva

LAB HEAD: Dr. I. Tarasova

PROVIDER: PXD022868 | Pride | 2022-06-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	LUM00925_VG.features.tsv	Tabular
	LUM00925_VG.raw	Raw
	LUM00925_VG_identipy_bruteforce_PSMs.tsv	Tabular
	LUM00925_VG_identipy_bruteforce_PSMs_full.tsv	Tabular
	LUM00925_VG_identipy_bruteforce_peptides.tsv	Tabular

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Publications

Multiomic Profiling Identified EGF Receptor Signaling as a Potential Inhibitor of Type I Interferon Response in Models of Oncolytic Therapy by Vesicular Stomatitis Virus.

Nikitina Anastasia S AS Lipatova Anastasia V AV Goncharov Anton O AO Kliuchnikova Anna A AA Pyatnitskiy Mikhail A MA Kuznetsova Ksenia G KG Hamad Azzam A Vorobyev Pavel O PO Alekseeva Olga N ON Mahmoud Marah M Shakiba Yasmin Y Anufrieva Ksenia S KS Arapidi Georgy P GP Ivanov Mark V MV Tarasova Irina A IA Gorshkov Mikhail V MV Chumakov Peter M PM Moshkovskii Sergei A SA

International journal of molecular sciences 20220508 9

Cancer cell lines responded differentially to type I interferon treatment in models of oncolytic therapy using vesicular stomatitis virus (VSV). Two opposite cases were considered in this study, glioblastoma DBTRG-05MG and osteosarcoma HOS cell lines exhibiting resistance and sensitivity to VSV after the treatment, respectively. Type I interferon responses were compared for these cell lines by integrative analysis of the transcriptome, proteome, and RNA editome to identify molecular factors dete ...[more]

PMID: 35563635

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