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In vivo protein-protein crosslinking in human lens through lys or protein N-terminus with Gln/Glu residues

ABSTRACT: In this study we identified protein-protein crosslinking through lys or protein N-terminus with Gln/Glu residues in aged human lens tissues. Such crosslinks could be reproduced experimentally by incubation of Glu- or Gln-containing peptides and their formation were consistent with an amino group attacking a glutarimide intermediate.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lens Fiber Cell, Lens

DISEASE(S): Nuclear Senile Cataract

SUBMITTER: Zhen Wang

LAB HEAD: Kevin L Schey

PROVIDER: PXD022991 | Pride | 2023-08-01

REPOSITORIES: Pride

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			Action	DRS
	ah_4068_ZW_032115_USF_IC_57yo.raw	Raw
	ah_4068_ZW_032115_USF_IC_57yo_HCDFT.cross-linked.LysGln.plabel	Other
	ah_4068_ZW_032115_USF_IC_57yo_HCDFT.cross-linked.LysGlu.plabel	Other
	ah_4068_ZW_032115_USF_N_57yo.raw	Raw
	ah_4068_ZW_032115_USF_N_57yo_HCDFT.cross-linked.LysGln.plabel	Other

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