Dataset Information

Molecular architecture of the endocytic TPLATE complex – part2 IP-MS dataset

ABSTRACT: Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET (TPLATE complex/TPC) using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generates novel evolutionary insight into the difference between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer-puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Seedling

SUBMITTER: Dominique Eeckhout

LAB HEAD: Daniel Van Damme

PROVIDER: PXD023051 | Pride | 2021-03-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Araport11plus.fasta	Fasta
	E22197b_A1_u_1p_PSB-237_1.raw	Raw
	E22199b_A1_u_1p_PSB-237_2.raw	Raw
	E22201b_A1_u_1p_PSB-237_3.raw	Raw
	E22203b_A1_u_1p_PSB-237_4.raw	Raw

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Publications

Molecular architecture of the endocytic TPLATE complex.

Yperman Klaas K Wang Jie J Eeckhout Dominique D Winkler Joanna J Vu Lam Dai LD Vandorpe Michael M Grones Peter P Mylle Evelien E Kraus Michael M Merceron Romain R Nolf Jonah J Mor Eliana E De Bruyn Pieter P Loris Remy R Potocký Martin M Savvides Savvas N SN De Rybel Bert B De Jaeger Geert G Van Damme Daniël D Pleskot Roman R

Science advances 20210226 9

Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as an endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET [TPLATE complex (TPC)] using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and memb ...[more]

PMID: 33637534

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