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SILAC phosphoproteome analysis upon transient Abl1/2 or DDR1 knockdown in human U-2 OS cells

ABSTRACT: Analysis of changes in the phosphoproteome upon transient siRNA-mediated knockdown of ABL1/ABL2 or DDR1 for 48 hours. CILAC phosphoproteome analysis was conducted after 48hrs using human U-2 OS cells.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): U2-os Cell

SUBMITTER: Nicolas Nalpas

LAB HEAD: Prof. Dr. Tassula Proikas-Cezanne

PROVIDER: PXD023146 | Pride | 2023-10-24

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20180702_CO_0810TaPC_Mix2_R4R5_phospho_1.raw	Raw
	20180702_CO_0810TaPC_Mix2_R4R5_phospho_10.raw	Raw
	20180702_CO_0810TaPC_Mix2_R4R5_phospho_2.raw	Raw
	20180702_CO_0810TaPC_Mix2_R4R5_phospho_3.raw	Raw
	20180702_CO_0810TaPC_Mix2_R4R5_phospho_4.raw	Raw

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Publications

The ABL-MYC axis controls WIPI1-enhanced autophagy in lifespan extension.

Sporbeck Katharina K Haas Maximilian L ML Pastor-Maldonado Carmen J CJ Schüssele David S DS Hunter Catherine C Takacs Zsuzsanna Z Diogo de Oliveira Ana L AL Franz-Wachtel Mirita M Charsou Chara C Pfisterer Simon G SG Gubas Andrea A Haller Patricia K PK Knorr Roland L RL Kaulich Manuel M Macek Boris B Eskelinen Eeva-Liisa EL Simonsen Anne A Proikas-Cezanne Tassula T

Communications biology 20230824 1

Human WIPI β-propellers function as PI3P effectors in autophagy, with WIPI4 and WIPI3 being able to link autophagy control by AMPK and TORC1 to the formation of autophagosomes. WIPI1, instead, assists WIPI2 in efficiently recruiting the ATG16L1 complex at the nascent autophagosome, which in turn promotes lipidation of LC3/GABARAP and autophagosome maturation. However, the specific role of WIPI1 and its regulation are unknown. Here, we discovered the ABL-ERK-MYC signalling axis controlling WIPI1. ...[more]

PMID: 37620393

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2019-03-20 | PXD011899 | Pride

Linking post-translational modifications and protein turnover by site-resolved protein turnover (SPOT) profiling

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