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Neuropeptides in Lineus longissimus larvae

ABSTRACT: The goal of this project was to identify potential neuropeptides in extracts from larvae of the nemertean species Lineus longissimus.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Lineus Longissimus

SUBMITTER: Nicolas Nalpas

LAB HEAD: Andreas Hejnol

PROVIDER: PXD023147 | Pride | 2022-02-16

REPOSITORIES: Pride

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			Action	DRS
	20150722_CO_0556DaTh_R02.raw	Raw
	txt.zip	Other

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Publications

Nemertean, Brachiopod, and Phoronid Neuropeptidomics Reveals Ancestral Spiralian Signaling Systems.

Thiel Daniel D Yañez-Guerra Luis A LA Franz-Wachtel Mirita M Hejnol Andreas A Jékely Gáspár G

Molecular biology and evolution 20211001 11

Neuropeptides are diverse signaling molecules in animals commonly acting through G-protein coupled receptors (GPCRs). Neuropeptides and their receptors underwent extensive diversification in bilaterians and the relationships of many peptide-receptor systems have been clarified. However, we lack a detailed picture of neuropeptide evolution in lophotrochozoans as in-depth studies only exist for mollusks and annelids. Here, we analyze peptidergic systems in Nemertea, Brachiopoda, and Phoronida. We ...[more]

PMID: 34272863

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Project description:Purpose: The goals of this study are to determine effects of castration on gene expression in longissimus dorsi muscle by comparing transcriptome profiles and to search candidate genes related with beef quality like flavor, tenderness, juiciness and fat deposition Methods: longissimus dorsi muscle mRNA profiles of 3 bulls and 3 steers of Korean cattles were generated by RNA sequencing using Illumina NextSeq 500. After quality checking, Tophat2 software was used for read mapping, and EdgeR was used to identify differentially expressed genes (DEGs) between bulls and steers. Gene ontology pathway analysis on DEGs was conducted with DAVID tool for categorization of DEGs. Results: Using an optimized data analysis workflow, we mapped about 58 million sequence reads per sample to the bovine genome (build UMD3.1) and identified 18,027 expressed genes in the longissimus dorsi muscle of bulls and steers with TopHat2 workflow. RNA-seq data confirmed 1,146 differentially expressed genes (adjusted p-value, FDR <0.05). Conclusions: We comparatively analyzed the transcriptome profile from longissimus dorsi muscle of bulls and steers of Korean cattles using NGS and identified DEGs between bulls and steers. The functional annotation analysis of DEGs found that transcriptome profile difference in longissimus dorsi muscle by castration.

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