Proteomics

Dataset Information

0

Genetic manipulation of Hmr leads to altered protein-protein interactions network


ABSTRACT: In this study we use Affinity Purification coupled with Mass Spectrometry (AP-MS) to test the effect of HMR overexpression (Hmr.over) or mutation (Hmr.dC and Hmr.2) on the interactome of HMR and in relation to the Speciation Core Complex (SCC). All affinity purifications were performed with a FLAG antibody to target either the exogenously expressed transgenes (Hmr.over, Hmr.dC, Hmr.2) or an endogenously FLAG-tagged HMR (Hmr.endo). Hmr.dC mutants carry a truncation in the BESS-domain-containing C-terminus of HMR. Hmr.2 carries 2 point mutations towards its N-terminus.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture

SUBMITTER: Andrea Lukacs  

LAB HEAD: Axel Imhof

PROVIDER: PXD023193 | Pride | 2021-08-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
ALMS288_CTRL_FLAG_REF1227.raw Raw
ALMS289_HMR.endo_FLAG_REF1227_.raw Raw
ALMS294_HMR.over_FLAG_REF1227.raw Raw
ALMS296_HMR.dc_FLAG_REF1227.raw Raw
ALMS298_HMR.over_FLAG_REF1227.raw Raw
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