Proteomics

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Multiplexed complexome profiling using tandem mass tags

ABSTRACT: Complexome profiling is a rapidly spreading, powerful approach to gain insight into the nature of protein complexes. It identifies and quantifies protein complexes separated into multiple fractions of increasing molecular mass using mass spectrometry-based, label-free bottom-up proteomics. Complexome profiling enables a sophisticated and thorough characterization of the composition, molecular mass, assembly and interactions of protein complexes. However, in practice, its application is limited by the large number of samples it generates and the related time and costs of mass spectrometry analyses. Here we implemented tandem mass tags for multiplexing complexome profiling.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Christoph Krisp  

LAB HEAD: Sergio Guerrero-Castillo

PROVIDER: PXD023211 | Pride | 2021-08-20

REPOSITORIES: Pride

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Multiplexed complexome profiling using tandem mass tags.

Guerrero-Castillo Sergio S   Krisp Christoph C   Küchler Katrin K   Arnold Susanne S   Schlüter Hartmut H   Gersting Søren W SW  

Biochimica et biophysica acta. Bioenergetics 20210518 9

Complexome profiling is a rapidly spreading, powerful technique to gain insight into the nature of protein complexes. It identifies and quantifies protein complexes separated into multiple fractions of increasing molecular mass using mass spectrometry-based, label-free bottom-up proteomics. Complexome profiling enables a sophisticated and thorough characterization of the composition, molecular mass, assembly, and interactions of protein complexes. However, in practice, its application is limited  ...[more]

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