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Large-scale LC-MS/MS identifiection of interactors of the Arabidopsis plant natriuretic peptide, AtPNP-A

ABSTRACT: Plant natriuretic peptides (PNPs) are systemically mobile peptidic hormones regulating solute and water homeostasis. Since the molecular mode of their action is elusive, we performed a large-scale protein-protein interaction study to identify putative interactors of AtPNP-A - the model PNP present in Arabidopsis thaliana.

INSTRUMENT(S): LTQ Orbitrap Velos, LTQ Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Ilona Turek

LAB HEAD: Chris Gehring

PROVIDER: PXD023216 | Pride | 2021-09-09

REPOSITORIES: Pride

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			Action	DRS
	A_thaliana_AC_MS20121003_term_11__F001671_.mzid.gz	Mzid
	A_thaliana_AC_MS20121003_term_11__F001671_.mzid.gz_A_thaliana_AC_MS20121003_term_11__F001671_.MGF	Mzid
	A_thaliana_AC_MS20121003_term_13__F001673_.mzid.gz	Mzid
	A_thaliana_AC_MS20121003_term_13__F001673_.mzid.gz_A_thaliana_AC_MS20121003_term_13__F001673_.MGF	Mzid
	A_thaliana_AC_MS20121003_term_17__F001677_.mzid.gz	Mzid

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Publications

Arabidopsis Plant Natriuretic Peptide Is a Novel Interactor of Rubisco Activase.

Turek Ilona I Gehring Chris C Irving Helen H

Life (Basel, Switzerland) 20201231 1

Plant natriuretic peptides (PNPs) are a group of systemically acting peptidic hormones affecting solute and solvent homeostasis and responses to biotrophic pathogens. Although an increasing body of evidence suggests PNPs modulate plant responses to biotic and abiotic stress, which could lead to their potential biotechnological application by conferring increased stress tolerance to plants, the exact mode of PNPs action is still elusive. In order to gain insight into PNP-dependent signalling, we ...[more]

PMID: 33396438

Similar Datasets

LC-MS/MS identification of interactors of the plant natriuretic peptide, AtPNP-A

Project description:Plant natriuretic peptides (PNPs) comprise a class of systemically mobile molecules functioning as hormonal regulators of ion and fluid balance. Since the molecular mode of action of PNPs remains unclear, a large-scale protein-protein interaction study has been undertaken to identify interactors of the model PNP present in Arabidopsis thaliana, termed AtPNP-A.

2020-04-07 | PXD017925 | Pride

Plant Natriuretic Peptides induce a specific set of proteins diagnostic for an adaptive response to abiotic stress

Project description:In plants, structural and physiological evidence has suggested the presence of biologically active natriuretic peptides (PNPs). PNPs are secreted into the apoplast, are systemically mobile and elicit a range of responses signaling via cGMP. The PNP-dependent responses include tissue specific modifications of cation transport and changes in stomatal conductance and the photosynthetic rate. PNP also has a critical role in host defense responses. Surprisingly, PNP-homologues are also produced by several plant pathogens during host colonization suppressing host defense responses. Here we show that a synthetic peptide representing the biologically active fragment of the Arabidopsis thaliana PNP (AtPNP-A) induces the production of reactive oxygen species in suspension-cultured A. thaliana (Col-0) cells.

2014-11-20 | PXD001386 | Pride

The influence of long-term use of diet enriched with biologically active substances on transcirptomic profile in rats liver

Project description:The aim of the study was to identify genes which are differentially expressed in the liver from rat fed with control diet and rat fed with diet enriched with biologicaly active compounds after 3 and 14 months of experiment RNA from rats fed with control diet and rats fed with diet enriched with biologicaly active compunds were hybridized to Agilent two color microarrays with a common reference

2013-10-25 | E-GEOD-51657 | biostudies-arrayexpress

Effect of hPNPase on Escherichia coli transcritome

Project description:We replaced the natural pnp locus with the human cDNA and studied the transcriptomes of 3 strains, namely the wt pnp+ (C-1a), the mutant with pnp ORF deletion (C-5691) and the strain with the substitution of the bacterial ORF with the human one (C-6001).

2023-08-08 | GSE211960 | GEO

Effect of hPNPase on Escherichia coli transcritome after addition of rifampicin

2023-08-08 | GSE221825 | GEO

Purine nucleoside phosphorylase enables dual metabolic checkpoints that prevent T cell immunodeficiency and TLR7-associated autoimmunity

Project description:Purine nucleoside phosphorylase (PNP) enables the breakdown and recycling of guanine nucleosides. PNP insufficiency in humans is paradoxically associated with both immunodeficiency and autoimmunity, but the mechanistic basis for these outcomes is incompletely understood. Here we identify two immune lineage-dependent consequences of PNP inactivation dictated by distinct gene interactions. During T cell development, PNP inactivation is synthetically lethal with down-regulation of the dNTP triphosphohydrolase SAMHD1. This interaction requires deoxycytidine kinase activity and is antagonized by microenvironmental deoxycytidine. In B lymphocytes and macrophages, PNP regulates Toll like receptor 7 signaling by controlling the levels of its (deoxy)guanosine nucleoside ligands. Overriding this regulatory mechanism promotes germinal center formation in the absence of exogenous antigen and accelerates disease in a mouse model of autoimmunity. This work reveals that one purine metabolism gene protects against immunodeficiency and autoimmunity via independent mechanisms operating in distinct immune lineages and identifies PNP as a novel metabolic immune checkpoint.

2022-06-02 | GSE203003 | GEO

A diet enriched with biologically active substances as a protecting factor against carcinogenesis process induced by 1,2-dimethylhydrazine in rat liver

Project description:The aim of this study was to evaluate the ability of a diet enriched with biologically active compounds to protect against 1,2-dimethylhydrazine - induced carcinogenesis in 14-month old rats liver. Rats from control and experimental groups after 14 months of experiment were given 5 times 1,2-dimethylhydrazine (DMH) by intraperitoneal treatment at a dose of 30mg/kg of body weight once a week to induce the process of carcinogenesis. RNA from their livers were hybridized to Agilent two color microarrays with a common reference. Then, the transcriptomic profile of these livers were compared to the transcriptomic profile of 14-month rats received the same control diet and diet enriched with biologically active substances, but without 1,2-dimethylhydrazine - induction (data from GEO Submission - GSE51657).

2014-02-27 | E-GEOD-55378 | biostudies-arrayexpress

Forward and reverse degradomics defines the proteolytic landscape of human knee osteoarthritic cartilage and the role of the serine protease HtrA1

Project description:Proteolytic destruction of articular cartilage is a major pathogenic mechanism in osteoarthritis (OA), but was not previously investigated on a proteome-wide scale. We sought to define the human knee OA cartilage degradome and the contribution therein of the serine protease HtrA1, using an N-terminomics strategy, terminal amine isotopic labeling of substrates (TAILS).

2023-11-03 | PXD028320 | Pride

A comprehensive and universal method for assessing the performance of differential gene expression analyses

Project description:The number of methods for pre-processing and analysis of gene expression data continues to increase, often making it difficult to select the most appropriate approach. We present a simple procedure for the comparative estimation of a variety of methods for microarray data pre-processing and analysis. Our approach is based on the use of real microarray data in which controlled fold changes are introduced into 20% of the data to provide a metric for comparison with the unmodified data. The data modification can be easily applied to raw data measured with any technological platform and retains all the complex structures and statistical characteristics of the real-world data. The power of the method is illustrated by its application to a comparative analysis of the significance analysis of microarray (SAM), Limma, and associative analysis tuned to the exact structure of the experimental data. We present a novel finding that SAM and Limma analyses fail to detect the most interesting differentially expressed genes at high expression level, while the associative analysis does recognize them. Our results demonstrate that the method of controlled modifications of real experimental data provides a simple tool for assessing the performance of data preprocessing and analysis methods. 20 samples of Epstein-Barr Virus-transformed B cells collected from normal healthy donors. All samples were biological. No treatment were introduced, and the whole group of 20 samples is presumably homogeneous

2010-11-09 | E-GEOD-22630 | biostudies-arrayexpress

Transcription profiling by array of Staphylococcus aureus wild type and rnc, pnp and sigB mutants

Project description:Transcriptome mapping of Staphylococcus aureus wild type and rnc, pnp and sigB mutants

2010-12-31 | E-MEXP-2778 | biostudies-arrayexpress