Dataset Information

SWATH-MS and MRM: Quantification of Ras-related proteins in HIV-1 Infected and Methamphetamine-Exposed Human Monocyte-Derived Macrophages (hMDM)

ABSTRACT: Macrophages are involved in the immunological response to inflammatory and infectious agents, including Human Immunodeficiency Virus type 1 (HIV-1) infection. They are a primary targets of the virus and facilitate transfer across the blood brain barrier into the central nervous system. In the presence of toxic substances, such as methamphetamine (Meth), the protective functions of macrophages are further impaired. It has been shown that both HIV-1 infection and Meth induce changes in the proteome of macrophages. Therefore, in the presented study we employed a nano-liquid chromatography (nanoLC)-SWATH mass spectrometry (MS) approach to detect and quantify changes on proteome level that are induced in human monocyte derived macrophages (hMDM) that were exposed to Meth before or after HIV infection. As we focused in this study on actin and a cluster of Ras-related proteins (Rab), we selected the following proteins: actin (UniProt P60709), Ras-related protein Rab-1A (UniProt P62820), Ras-related protein Rab-2A (UniProt P61019), Ras-related protein Rab-4B (UniProt P61018), Ras-related protein Rab-7A (UniProt P51149), Ras-related protein Rab-9A (UniProt P51151), Ras-related protein Rab-11 (UniProt P62491), Ras-related protein Rab-14 (UniProt P61106), Ras-related protein Rab-22A (UniProt Q9UL26), Ras-related protein Rab-24 (UniProt Q969Q5), Ras-related protein Rab-37 (UniProt Q96AX2) for further validation, which we performed using liquid chromatography-multiple reaction monitoring (LC-MRM) MS technique. The MRM data from this study, along with LC-MRM analysis and data processing parameters, were separately described and deposited in PASSEL repository with accession number PASS01591.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood, Macrophage

DISEASE(S): Disease Free,Human Immunodeficiency Virus Infectious Disease

SUBMITTER: Katarzyna Macur

LAB HEAD: Prof. Pawel Ciborowski

PROVIDER: PXD023291 | Pride | 2022-05-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2020-10-16-CIC-CIM-MIM_donors_pool_1.wiff	Wiff
	2020-10-16-CIC-CIM-MIM_donors_pool_1.wiff.scan	Wiff
	2020-10-30-CIC-CIM-MIM_donors_pool.wiff	Wiff
	2020-10-30-CIC-CIM-MIM_donors_pool.wiff.scan	Wiff
	2020-11-02-CIC-CIM-MIM_donors_pool_1.wiff	Wiff

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Publications

HIV-1 and methamphetamine alter galectins -1, -3, and -9 in human monocyte-derived macrophages.

Grabowska Kinga K Macur Katarzyna K Zieschang Sarah S Zaman Lubaba L Haverland Nicole N Schissel Andrew A Morsey Brenda B Fox Howard S HS Ciborowski Pawel P

Journal of neurovirology 20220217 1

Macrophages are key elements of the innate immune system. Their HIV-1 infection is a complex process that involves multiple interacting factors and various steps and is further altered by exposure of infected cells to methamphetamine (Meth), a common drug of abuse in people living with HIV. This is reflected by dynamic changes in the intracellular and secreted proteomes of these cells. Quantification of these changes poses a challenge for experimental design and associated analytics. In this stu ...[more]

PMID: 35175539

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