Dataset Information

Proteomic Analysis of Xinjiang Black Bee's Royal Jelly Using the Osboren Classification Method

ABSTRACT: Royal jelly has long been recognized as health food, with a high content of proteins. These proteins play important roles in honeybee caste and human health, but the proteomic analysis of those low-abundance proteins in royal jelly is always a challenge. Herein, we used the Osboren classification method to separate the royal jelly proteins of Xinjiang black bees, a sub-species of Apis mellifera mellifera, into various fractions. The globulin, ethanol-soluble protein and glutelin fractions were further separated by SDS-PAGE, and proteomic analysis was carried out by LC-MS/MS and searching against the NCBI database. A total of 63 proteins with definitive names were identified, in which 41 proteins were identified for the first time in royal jelly. The Osboren classification method combined with one-dimensional gel electrophoresis based proteomic analysis allows the identification of low-abundance proteins, and greatly extends the knowledge about the components and functions of royal jelly proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Apis Mellifera (honeybee)

SUBMITTER: Long Chen

LAB HEAD: Long Chen

PROVIDER: PXD023315 | Pride | 2024-08-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Ethanol-soluble_proteins-1.raw	Raw
	Ethanol-soluble_proteins-1.xlsx	Xlsx
	Ethanol-soluble_proteins-2.raw	Raw
	Ethanol-soluble_proteins-2.xlsx	Xlsx
	Ethanol-soluble_proteins-3.raw	Raw

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Publications

Identification of low-abundance proteins in the royal jelly using the Osborne classification method.

Pei Pengfei P Guo Wenxu W Qi Mingxue M Jiang Jianhui J Feng Shan S Luo Shi-Zhong SZ Chen Long L

Journal of proteomics 20240723

Royal jelly (RJ) is recognized as healthy food, with a high content of proteins. These proteins play important roles in honeybee caste and human health, but the proteomic analysis of low-abundance proteins in RJ has long been a challenge. Herein, we used the Osborne classification method to separate the RJ proteins of Xinjiang black bees into various fractions. The globulin, ethanol-soluble protein, and glutelin fractions were further separated by SDS-PAGE, and proteomic analysis was carried out ...[more]

PMID: 39053808

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