Proteomics

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Proteomic Analysis of Xinjiang Black Bee's Royal Jelly Using the Osboren Classification Method

ABSTRACT: Royal jelly has long been recognized as health food, with a high content of proteins. These proteins play important roles in honeybee caste and human health, but the proteomic analysis of those low-abundance proteins in royal jelly is always a challenge. Herein, we used the Osboren classification method to separate the royal jelly proteins of Xinjiang black bees, a sub-species of Apis mellifera mellifera, into various fractions. The globulin, ethanol-soluble protein and glutelin fractions were further separated by SDS-PAGE, and proteomic analysis was carried out by LC-MS/MS and searching against the NCBI database. A total of 63 proteins with definitive names were identified, in which 41 proteins were identified for the first time in royal jelly. The Osboren classification method combined with one-dimensional gel electrophoresis based proteomic analysis allows the identification of low-abundance proteins, and greatly extends the knowledge about the components and functions of royal jelly proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Apis Mellifera (honeybee)

SUBMITTER: Long Chen  

LAB HEAD: Long Chen

PROVIDER: PXD023315 | Pride | 2024-08-09

REPOSITORIES: Pride

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Publications

Identification of low-abundance proteins in the royal jelly using the Osborne classification method.

Pei Pengfei P   Guo Wenxu W   Qi Mingxue M   Jiang Jianhui J   Feng Shan S   Luo Shi-Zhong SZ   Chen Long L  

Journal of proteomics 20240723

Royal jelly (RJ) is recognized as healthy food, with a high content of proteins. These proteins play important roles in honeybee caste and human health, but the proteomic analysis of low-abundance proteins in RJ has long been a challenge. Herein, we used the Osborne classification method to separate the RJ proteins of Xinjiang black bees into various fractions. The globulin, ethanol-soluble protein, and glutelin fractions were further separated by SDS-PAGE, and proteomic analysis was carried out  ...[more]

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