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Unbiased characterization and systematic profiling of metabolically labeled RNA-binding sites

ABSTRACT: Here we introduce pRBS-ID, an amenable platform that enables robust profiling of PAR-crosslinked RBSs. Using tandem mass spectrometry (MS/MS) approaches, we unbiasedly characterize the PAR adducts on peptides, to develop an optimal MS/MS search pipeline that enables robust identification and quantification. Using pRBS-ID, we systematically profile RBSs crosslinked to U or G in PAR-labeled mRNA, which can serve as a rich resource for studying RNA-protein interactions. Especially, pRBS-ID is widely applicable to studies on time-course RNP remodelling, host-virus interactions, and RNP structure prediction.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Jong Woo Bae

LAB HEAD: Jong-Seo Kim

PROVIDER: PXD023401 | Pride | 2021-11-02

REPOSITORIES: Pride

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			Action	DRS
	GlobalRBS_dTTE_HF_150x10_1.raw	Raw
	GlobalRBS_dTTE_HF_150x10_1_MXFiltered.mgf	Mgf
	GlobalRBS_dTTE_HF_150x10_1_MXFiltered.mzid.gz	Mzid
	GlobalRBS_dTTE_HF_150x10_1_UMC.txt	Txt
	GlobalRBS_dTTE_HF_150x10_1_UMC_Components.txt	Txt

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