Dataset Information

Global mapping of RNA-binding proteins in multi-drug resistant Staphylococcus aureus using PTex

ABSTRACT: Phenol Toluol extraction (PTex) carried out two steps organic-phase separation: first pH neutral phenol–Toluol extraction separated RNA and protein from DNA: RNA and proteins to the top aqueous phase but DNA and membranes in the interface. Aqueous phase then went through two rounds acid-phenol extraction, resulting RNA in the top aqueous phase, proteins bottom organic phase and RNA-protein complexes in the interface. After carefully isolated the interface, ethanol precipitation was carried on for purifying RNA-protein adducts (Urdaneta et al, 2019; Smith et al, 2020)

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Staphylococcus Aureus

TISSUE(S): Cell Culture

SUBMITTER: Liangcui Chu

LAB HEAD: Sander Granneman

PROVIDER: PXD023414 | Pride | 2022-05-31

REPOSITORIES: Pride

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Publications

The RNA-bound proteome of MRSA reveals post-transcriptional roles for helix-turn-helix DNA-binding and Rossmann-fold proteins.

Chu Liang-Cui LC Arede Pedro P Li Wei W Urdaneta Erika C EC Ivanova Ivayla I McKellar Stuart W SW Wills Jimi C JC Fröhlich Theresa T von Kriegsheim Alexander A Beckmann Benedikt M BM Granneman Sander S

Nature communications 20220524 1

RNA-binding proteins play key roles in controlling gene expression in many organisms, but relatively few have been identified and characterised in detail in Gram-positive bacteria. Here, we globally analyse RNA-binding proteins in methicillin-resistant Staphylococcus aureus (MRSA) using two complementary biochemical approaches. We identify hundreds of putative RNA-binding proteins, many containing unconventional RNA-binding domains such as Rossmann-fold domains. Remarkably, more than half of the ...[more]

PMID: 35610211

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