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Nanobodies peptide/glycopeptide identification

ABSTRACT: Confirmation of Nanobody sequences and identification of Nanobody glycopeptide glycoforms.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Lama Glama

SUBMITTER: Nichollas Scott

LAB HEAD: Associate Professor Wai-Hong Tham

PROVIDER: PXD023483 | Pride | 2021-09-09

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	Nsco_1482_WHT_Byonic.zip	Other
	Nsco_1482_WHT_WNB_15_FC.raw	Raw
	Nsco_1482_WHT_WNB_2_FC.raw	Raw
	Nsco_1482_WHT_WNB_36_FC.raw	Raw
	Nsco_1482_WHT_WNB_7_FC.raw	Raw

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Publications

Nanobody cocktails potently neutralize SARS-CoV-2 D614G N501Y variant and protect mice.

Pymm Phillip P Adair Amy A Chan Li-Jin LJ Cooney James P JP Mordant Francesca L FL Allison Cody C CC Lopez Ester E Haycroft Ebene R ER O'Neill Matthew T MT Tan Li Lynn LL Dietrich Melanie H MH Drew Damien D Doerflinger Marcel M Dengler Michael A MA Scott Nichollas E NE Wheatley Adam K AK Gherardin Nicholas A NA Venugopal Hariprasad H Cromer Deborah D Davenport Miles P MP Pickering Raelene R Godfrey Dale I DI Purcell Damian F J DFJ Kent Stephen J SJ Chung Amy W AW Subbarao Kanta K Pellegrini Marc M Glukhova Alisa A Tham Wai-Hong WH

Proceedings of the National Academy of Sciences of the United States of America 20210501 19

Neutralizing antibodies are important for immunity against SARS-CoV-2 and as therapeutics for the prevention and treatment of COVID-19. Here, we identified high-affinity nanobodies from alpacas immunized with coronavirus spike and receptor-binding domains (RBD) that disrupted RBD engagement with the human receptor angiotensin-converting enzyme 2 (ACE2) and potently neutralized SARS-CoV-2. Epitope mapping, X-ray crystallography, and cryo-electron microscopy revealed two distinct antigenic sites a ...[more]

PMID: 33893175

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