Proteomics

Dataset Information

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HyperLOPIT and temporal proteomic profiling of the response to lipopolysaccharide in the THP-1 human leukaemia cell line


ABSTRACT: The hyperLOPIT proteomics platform combines mass-spectrometry with state-of-the-art machine learning for simultaneous “mapping” of the steady-state subcellular location of thousands of proteins. Here, we use a synergistic approach and combine global proteome analysis with hyperLOPIT in a fully Bayesian framework to elucidate the spatio-temporal changes that occur during the pro-inflammatory response to lipopolysaccharide in the human monocytic leukaemia cell-line THP-1. We report cell-wide protein relocalisations upon LPS stimulation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, Blood

SUBMITTER: Claire Mulvey  

LAB HEAD: Kathryn Susan Lilley

PROVIDER: PXD023509 | Pride | 2022-02-16

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Claire_macrophage_TC_replicate3_newparam.msf Msf
LPS_REP1_SET1_131117.msf Msf
LPS_REP1_SET2_131117.msf Msf
LPS_REP2_SET1_131117.msf Msf
LPS_REP2_SET2_131117.msf Msf
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Publications

Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line.

Mulvey Claire M CM   Breckels Lisa M LM   Crook Oliver M OM   Sanders David J DJ   Ribeiro Andre L R ALR   Geladaki Aikaterini A   Christoforou Andy A   Britovšek Nina Kočevar NK   Hurrell Tracey T   Deery Michael J MJ   Gatto Laurent L   Smith Andrew M AM   Lilley Kathryn S KS  

Nature communications 20211001 1


Protein localisation and translocation between intracellular compartments underlie almost all physiological processes. The hyperLOPIT proteomics platform combines mass spectrometry with state-of-the-art machine learning to map the subcellular location of thousands of proteins simultaneously. We combine global proteome analysis with hyperLOPIT in a fully Bayesian framework to elucidate spatiotemporal proteomic changes during a lipopolysaccharide (LPS)-induced inflammatory response. We report a hi  ...[more]

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